Gene targeting restricted to mouse striated muscle lineage

被引：165

作者：

Miniou, Pierre ^{[1
]}

Tiziano, Danilo ^{[1
]}

Frugier, Tony ^{[1
]}

Roblot, Natacha ^{[1
]}

Le Meur, Marianne ^{[1
]}

Melki, Judith ^{[1
]}

机构：

[1] CU Strasbourg, CNRS, INSERM, ULP,IGBMC, F-67404 Illkirch Graffenstaden, France

来源：

NUCLEIC ACIDS RESEARCH | 1999年 / 27卷 / 19期

关键词：

D O I：

10.1093/nar/27.19.e27

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spatially and temporally regulated somatic mutations can be achieved by using the Cre/LoxP recombination system of bacteriophage P1. In order to develop gene knockouts restricted to striated muscle, we generated a transgenic mouse line expressing Cre recombinase under the control of the human alpha-skeletal actin promoter. Specific excision of a loxP-flanked gene was demonstrated in striated muscle, heart and skeletal muscle, in a pattern very similar to the expression of the endogenous alpha-skeletal actin gene. Therefore, the reported transgenic line can be used to target inactivation or activation of a given gene to the skeletal muscle lineage.

引用

页数：4