Crystal Structure of the Neisseria gonorrhoeae MtrD Inner Membrane Multidrug Efflux Pump

被引：55

作者：

Bolla, Jani Reddy ^{[1
]}

Su, Chih-Chia ^{[2
]}

Do, Sylvia V. ^{[3
]}

Radhakrishnan, Abhijith ^{[1
]}

Kumar, Nitin ^{[1
]}

Long, Feng ^{[2
]}

Chou, Tsung-Han ^{[2
]}

Delmar, Jared A. ^{[2
]}

Lei, Hsiang-Ting ^{[1
]}

Rajashankar, Kanagalaghatta R. ^{[4
,5
]}

Shafer, William M. ^{[6
,7
]}

Yu, Edward W. ^{[1
,2
,3
]}

机构：

[1] Iowa State Univ, Dept Chem, Ames, IA 50011 USA

[2] Iowa State Univ, Dept Phys & Astron, Ames, IA USA

[3] Iowa State Univ, Bioinformat & Computat Biol Interdept Grad Progra, Ames, IA USA

[4] NE CAT, Argonne, IL USA

[5] Cornell Univ, Argonne Natl Lab, Dept Chem & Chem Biol, Argonne, IL USA

[6] Emory Univ, Sch Med, Dept Microbiol & Immunol, Atlanta, GA 30322 USA

[7] VA Med Ctr, Labs Microbial Pathogenesis, Decatur, GA USA

来源：

PLOS ONE | 2014年 / 9卷 / 06期

基金：

美国国家卫生研究院;

关键词：

ESCHERICHIA-COLI; PSEUDOMONAS-AERUGINOSA; TRANSPORTER ACRB; FUSION PROTEIN; BINDING POCKET; SYSTEM; RESISTANCE; MECHANISM; SOFTWARE; CHANNEL;

D O I：

10.1371/journal.pone.0097903

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Neisseria gonorrhoeae is an obligate human pathogen and the causative agent of the sexually-transmitted disease gonorrhea. The control of this disease has been compromised by the increasing proportion of infections due to antibiotic-resistant strains, which are growing at an alarming rate. The MtrCDE tripartite multidrug efflux pump, belonging to the hydrophobic and amphiphilic efflux resistance-nodulation-cell division (HAE-RND) family, spans both the inner and outer membranes of N. gonorrhoeae and confers resistance to a variety of antibiotics and toxic compounds. We here report the crystal structure of the inner membrane MtrD multidrug efflux pump, which reveals a novel structural feature that is not found in other RND efflux pumps.

引用

页数：8

共 38 条

[31] Crystal structure of the CusBA heavy-metal efflux complex of Escherichia coli [J].