Crystal Structure of the Neisseria gonorrhoeae MtrD Inner Membrane Multidrug Efflux Pump

被引：55
作者：
Bolla, Jani Reddy [1 ]
Su, Chih-Chia [2 ]
Do, Sylvia V. [3 ]
Radhakrishnan, Abhijith [1 ]
Kumar, Nitin [1 ]
Long, Feng [2 ]
Chou, Tsung-Han [2 ]
Delmar, Jared A. [2 ]
Lei, Hsiang-Ting [1 ]
Rajashankar, Kanagalaghatta R. [4 ,5 ]
Shafer, William M. [6 ,7 ]
Yu, Edward W. [1 ,2 ,3 ]
机构：
[1] Iowa State Univ, Dept Chem, Ames, IA 50011 USA
[2] Iowa State Univ, Dept Phys & Astron, Ames, IA USA
[3] Iowa State Univ, Bioinformat & Computat Biol Interdept Grad Progra, Ames, IA USA
[4] NE CAT, Argonne, IL USA
[5] Cornell Univ, Argonne Natl Lab, Dept Chem & Chem Biol, Argonne, IL USA
[6] Emory Univ, Sch Med, Dept Microbiol & Immunol, Atlanta, GA 30322 USA
[7] VA Med Ctr, Labs Microbial Pathogenesis, Decatur, GA USA
来源：
PLOS ONE | 2014年 / 9卷 / 06期
基金：
美国国家卫生研究院;
关键词：
ESCHERICHIA-COLI; PSEUDOMONAS-AERUGINOSA; TRANSPORTER ACRB; FUSION PROTEIN; BINDING POCKET; SYSTEM; RESISTANCE; MECHANISM; SOFTWARE; CHANNEL;
D O I：
10.1371/journal.pone.0097903
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Neisseria gonorrhoeae is an obligate human pathogen and the causative agent of the sexually-transmitted disease gonorrhea. The control of this disease has been compromised by the increasing proportion of infections due to antibiotic-resistant strains, which are growing at an alarming rate. The MtrCDE tripartite multidrug efflux pump, belonging to the hydrophobic and amphiphilic efflux resistance-nodulation-cell division (HAE-RND) family, spans both the inner and outer membranes of N. gonorrhoeae and confers resistance to a variety of antibiotics and toxic compounds. We here report the crystal structure of the inner membrane MtrD multidrug efflux pump, which reveals a novel structural feature that is not found in other RND efflux pumps.
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页数：8
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