Ameliorating injury during preservation and isolation of human islets using the two-layer method with perfluorocarbon and UW solution

This study assessed the effects of a two-layer method (TLM), using perfluorocarbon and UW solution. on the quality of human pancreata following storage and islet yield/function after isolation. In part A, TLM was applied immediately after procurement and the energetic profile was compared to a group treated with UW solution only (control) throughout 24-h storage. In part B, cadaveric human pancreata were procured and subjected to a TLM after cold storage in UW solution (TLM group) or UW solution (control group). Energetics. lipid peroxidation. and islet recovery/function were assessed after preservation at 4 degrees C. In part A. after 9-h storage the energetic profile (ATP. ATP/ADP, energy charge) for the TLM group was superior to controls. In part B, TLM treatment resulted in consistently greater ATP, ATP/ADR and energy charge values than with storage in UW Solution alone (p < 0.05). UW treatment resulted in 40% greater peroxidative damage than in the TLM group (p < 0.05). Islet recovery and functional viability were 30-40% higher following TLM treatment (p < 0.05). These data support the hypothesis that islet viability and yields can be significantly improved using a brief period of TLM treatment following conventional UW storage: reduced energetic and oxidative stress are implicated as potential mechanisms.