Uptake Mechanism of Oppositely Charged Fluorescent Nanoparticles in HeLa Cells

被引：239

作者：

Dausend, Julia ^{[1
,2
]}

Musyanovych, Anna ^{[2
]}

Dass, Martin ^{[2
,3
]}

Walther, Paul ^{[3
]}

Schrezenmeier, Hubert ^{[1
]}

Landfester, Katharina ^{[2
,4
]}

Mailaender, Volker ^{[1
,4
]}

机构：

[1] Univ Ulm, Inst Clin Transfus Med & Immunogenet Ulm, Dept Transfus Med, D-89081 Ulm, Germany

[2] Univ Ulm, Inst Organ Chem Macromol Chem & Organ 3, D-89081 Ulm, Germany

[3] Univ Ulm, Cent Facil Elect Microscopy, D-89081 Ulm, Germany

[4] Max Planck Inst Polymer Res, D-55128 Mainz, Germany

来源：

MACROMOLECULAR BIOSCIENCE | 2008年 / 8卷 / 12期

关键词：

biological application of polymers; endocytosis; endocytosis inhibition; nanoparticles; TEM;

D O I：

10.1002/mabi.200800123

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The endocytotic mechanisms involved in the uptake of charged polystyrene nanoparticles into HeLa cells were investigated. Uptake experiments were done in the presence or absence of drugs known to inhibit various factors in endocytosis. Independent of the particle charge, endocytosis is highly dependent on F-actin, and tyrosine-specific protein kinases, which suggests a dynamin-dependent and lipid raft-dependent mechanism. However, cholesterol depletion did not hinder particle uptake. Regarding positively charged particles, macropinocytosis, the microtubule network, and cyclooxygenases are also involved. The clathrin-dependent pathway plays a minor role.

引用

页码：1135 / 1143

页数：9

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