A simple and sensitive electrochemical aptasensor for determination of Chloramphenicol in honey based on target-induced strand release

被引：73

作者：

Yan, Li ^{[1
]}

Luo, Caihui ^{[1
]}

Cheng, Wei ^{[2
]}

Mao, Wei ^{[3
]}

Zhang, Decai ^{[1
]}

Ding, Shijia ^{[1
]}

机构：

[1] Chongqing Med Univ, Coll Lab Med, Minist Educ China, Key Lab Clin Lab Diagnost, Chongqing 400016, Peoples R China

[2] Chongqing Med Univ, Affiliated Hosp 1, Mol Oncol & Epigenet Lab, Chongqing 400016, Peoples R China

[3] Chongqing Blood Ctr, Chongqing 400015, Peoples R China

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2012年 / 687卷

基金：

中国国家自然科学基金;

关键词：

Aptamer; Electrochemical biosensor; Chloramphenicol; LIQUID-CHROMATOGRAPHY; GAS-CHROMATOGRAPHY; FLORFENICOL AMINE; RESIDUES; THIAMPHENICOL; SHRIMP; IMMUNOSENSOR; EXTRACTION; BIOSENSOR; SENSORS;

D O I：

10.1016/j.jelechem.2012.10.016

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel electrochemical aptasensor for highly sensitive detection of Chloramphenicol (CAP) in honey was successfully developed based on target-induced strand release (TISR). CAP aptamer was immobilized on electrode and then hybridized with the complementary biotinylated detection probe to form aptamer/DNA duplex. In the presence of CAP, the TISR resulted in the dissociation of biotinylated detection probe from the electrode. Then the binding of streptavidin-alkaline phosphatase (ST-AP) to the remaining biotinylated detection probe led to enzyme-amplified electrochemical signal, which decreased with the increase of CAP concentration. Under optimal conditions, the electrochemical signal was linear with the logarithm of CAP concentrations in the range from 1 nM to 1000 nM with the detection limit of 0.29 nM of CAP. In addition, the designed strategy allowed the direct analysis of real honey samples and the results showed a good agreement with conventional LC-MS/MS method. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：89 / 94

页数：6

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