Development and in vitro characterization of insulin loaded whey protein and alginate microparticles

被引：37

作者：

Deat-Laine, Emmanuelle ^{[1
]}

Hoffart, Valerie ^{[1
]}

Cardot, Jean-Michel ^{[1
]}

Subirade, Muriel ^{[2
]}

Beyssac, Eric ^{[1
]}

机构：

[1] Univ Auvergne, UFR Pharm, ERT CIDAM, F-63001 Clermont Ferrand, France

[2] Univ Laval, Chaire Rech Canada Physicochim Prot Biosyst & Ali, INAF, Dept Sci Aliments & Nutr, Quebec City, PQ G1K 7P4, Canada

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2012年 / 439卷 / 1-2期

关键词：

Whey protein; Alginate; Insulin; Microparticle; Oral administration; GEL BEADS; DELIVERY; RELEASE; PROTECTION; HYDROGELS; CHITOSAN;

D O I：

10.1016/j.ijpharm.2012.10.003

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Insulin was encapsulated into microparticles (MP) made of denaturized whey proteins (WP) and alginate (ALG) using an extrusion/cold gelation process with calcium ions. High encapsulation efficiency of 85% was obtained. Influence of insulin on polymeric viscosity and on microparticle behavior was evaluated. Insulin seemed to interact with WP chains by non covalent binding and steric hindrance. This influence was balanced by ALG addition. Nevertheless, insulin was released rapidly by diffusion at both acidic and intestinal dissolution media. Despite this fast in vitro release, WP/ALG MP showed an important enzymatic inhibition effect on trypsin and alpha-chymotrypsin. Thus, WP/ALG MP contributed to an effective insulin protection towards enzymatic degradation. The aforementioned results suggested that WP based microparticles are a promising carrier for improving oral delivery of insulin. (C) 2012 Elsevier B. V. All rights reserved.

引用

页码：136 / 144

页数：9

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