Damaged muscle fibers might masquerade as hybrid fibers - a cautionary note on immunophenotyping mouse muscle with mouse monoclonal antibodies

被引：8

作者：

Begam, Morium ^{[1
]}

Roche, Joseph A. ^{[1
]}

机构：

[1] Wayne State Univ, Dept Hlth Care Sci, Phys Therapy Program, 259 Mack Ave,Room 4440, Detroit, MI 48201 USA

来源：

EUROPEAN JOURNAL OF HISTOCHEMISTRY | 2018年 / 62卷 / 03期

基金：

美国国家卫生研究院;

关键词：

Skeletal muscle; myosin heavy chains; fiber type; immunolabeling; hybrid muscle fibers; muscle fiber damage; muscle injury; ECCENTRIC EXERCISE; MYOFIBER DAMAGE; MECHANISMS; MYOSIN; INJURY; DESMIN; REPAIR;

D O I：

10.4081/ejh.2018.2896

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We report that, labeling mouse muscle tissue, with mouse monoclonal antibodies specific to slow or fast myosin heavy chain (sMyHC and fMyHC, respectively), can lead to artefactual labeling of damaged muscle fibers, as hybrid fibers (sMyHC+ and fMyHC+). We demonstrate that, such erroneous immunophenotyping of muscle may be avoided, by performing colabeling or serialsection-labeling, to identify damaged fibers. The quadriceps femoris muscle group (QF) in 7-month-old, male, C57BL/6J mice had: 1.21 +/- 0.21%, 98.34 +/- 1.06%, 0.07 +/- 0.01%, and 0.53 +/- 0.85% fibers, that were, sMyHC+, fMyHC+, hybrid, and damaged. respectively. All fibers in the tibialis anterior muscle (TA) of 3-month-old, male, C57BL/6J mice were fMyHC+; and at 3 days after injurious eccentric contractions, there was no fiber-type shift, but similar to 18% fibers were damaged.

引用

页码：247 / 252

页数：6

共 31 条

[1] Eccentric muscle damage: mechanisms of early reduction of force [J].

Allen, DG .

ACTA PHYSIOLOGICA SCANDINAVICA, 2001, 171 (03) :311-319

[2] Desmin cytoskeletal modifications after a bout of eccentric exercise in the rat [J].

Barash, IA ;

Peters, D ;

Fridén, J ;

Lutz, GJ ;

Lieber, RL .

AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 2002, 283 (04) :R958-R963

[3] Diltiazem improves contractile properties of skeletal muscle in dysferlin-deficient BLAJ mice, but does not reduce contraction-induced muscle damage [J].

Begam, Morium ;

Collier, Alyssa F. ;

Mueller, Amber L. ;

Roche, Renuka ;

Galen, Sujay S. ;

Roche, Joseph A. .

PHYSIOLOGICAL REPORTS, 2018, 6 (11)

[4] Sodium 4-phenylbutyrate reduces myofiber damage in a mouse model of Duchenne muscular dystrophy [J].

Begam, Morium ;

Abro, Valerie M. ;

Mueller, Amber L. ;

Roche, Joseph A. .

APPLIED PHYSIOLOGY NUTRITION AND METABOLISM, 2016, 41 (10) :1108-1111

[5] Rapid Determination of Myosin Heavy Chain Expression in Rat, Mouse, and Human Skeletal Muscle Using Multicolor Immunofluorescence Analysis [J].

Bloemberg, Darin ;

Quadrilatero, Joe .

PLOS ONE, 2012, 7 (04)

[6] INTERACTIONS BETWEEN MOTONEURONES AND MUSCLES IN RESPECT OF THE CHARACTERISTIC SPEEDS OF THEIR RESPONSES [J].

BULLER, AJ ;

ECCLES, JC ;

ECCLES, RM .

JOURNAL OF PHYSIOLOGY-LONDON, 1960, 150 (02) :417-439

[7]

DIAMANDIS EP, 1991, CLIN CHEM, V37, P625

[8] Muscle insulin sensitivity and glucose metabolism are controlled by the intrinsic muscle clock [J].

Dyar, Kenneth A. ;

Ciciliot, Stefano ;

Wright, Lauren E. ;

Bienso, Rasmus S. ;

Tagliazucchi, Guidantonio M. ;

Patel, Vishal R. ;

Forcato, Mattia ;

Paz, Marcia I. P. ;

Gudiksen, Anders ;

Solagna, Francesca ;

Albiero, Mattia ;

Moretti, Irene ;

Eckel-Mahan, Kristin L. ;

Baldi, Pierre ;

Sassone-Corsi, Paolo ;

Rizzuto, Rosario ;

Bicciato, Silvio ;

Pilegaard, Henriette ;

Blaauw, Bert ;

Schiaffino, Stefano .

MOLECULAR METABOLISM, 2014, 3 (01) :29-41

[9]

Gray H., 1918, ANATOMY HUMAN BODY

[10] Fluorophore-labeled myosin-specific antibodies simplify muscle-fiber phenotyping [J].

Gregorevic, Paul ;

Meznarich, Norman A. ;

Blankinship, Michael J. ;

Crawford, Robert W. ;

Chamberlain, Jeffrey S. .

MUSCLE & NERVE, 2008, 37 (01) :104-106

← 1 2 3 4 →