Preparation and evaluation of stainless-steel wide-bore packed capillary high performance liquid chromatographic columns

被引：0
作者：
Tang, YH [1 ]
Zhu, DQ [1 ]
Guan, YF [1 ]
机构：
[1] Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China
来源：
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2001年 / 29卷 / 10期
关键词：
packed capillary liquid chromatographic column; column preparation; column end structure; packing reservoir;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The zero-dead volume union ends and conical slurry reservoir for microbore columns were designed. A detailed procedure for the preparation of highly efficient and stable stainless-steel microbore columns with reversed-phase ODS is described. The influence of packing direction, packing solvent, slurry solvent, slurry concentration and packing pressure has been studied systematically. Columns with efficiency of 75,000/m (number of theoretical plate) were prepared reproducibly at 40MPa packing pressure and optimized conditions. The peak asymmetric factor was 1.03 similar to 1.10 for all the compounds tested.
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页码：1228 / 1232
页数：5
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