Physicochemical and Antioxidant Properties of Industrial Hemp Seed Protein Isolate Treated by High-Intensity Ultrasound

被引：39
作者：
Karabulut, Gulsah [1 ,2 ,3 ]
Feng, Hao [2 ]
Yemis, Oktay [1 ,3 ]
机构：
[1] Sakarya Univ, Fac Engn, Dept Food Engn, TR-54187 Sakarya, Turkey
[2] Illinois Univ Urbana Champaign, Dept Food Sci & Human Nutr, Urbana, IL 61801 USA
[3] Sakarya Univ, Res Dev & Applicat Ctr SARGEM, TR-54050 Sakarya, Turkey
来源：
PLANT FOODS FOR HUMAN NUTRITION | 2022年 / 77卷 / 04期
关键词：
Hemp seed; Ultrasound; Plant protein; Modification; Physicochemical properties; Non-thermal technologies; FUNCTIONAL-PROPERTIES; PHYSICAL-PROPERTIES; ENZYMOLYSIS;
D O I：
10.1007/s11130-022-01017-7
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Ultrasound is one of the non-thermal, green, and novel technologies used to functionalize plant proteins. We recently determined the optimum conditions of high-intensity ultrasound (HIUS) treatment for maximum solubility and investigated the functional properties of hemp seed protein isolate (HSPI) under the optimal conditions. In this study, we analyzed changes in primary, secondary, and tertiary structures, physical microstructures, thermal stability, and antioxidant capacity of ultrasound-applied hemp protein isolate (HSPI-HIUS). The free SH group content (+59%) and zeta potential (+25%) increased upon ultrasound treatment. The electrophoretic protein patterns of HSPI showed no significant change after HIUS treatment. The FTIR spectrum revealed the wavenumber shifts in Amid 1 and 2 regions of protein. The denaturation temperature and the ratio of beta-structure increased after sonication. Antioxidant properties of hemp seed protein isolates were increased by 38% by ultrasound treatment. The obtained data in this study showed that HIUS treatment would be promising for improving the functional, physicochemical, and antioxidant properties of HSPI.
引用
收藏
页码：577 / 583
页数：7
相关论文
共 28 条
[1]   Comparative study of high intensity ultrasound effects on food proteins functionality [J].
Arzeni, C. ;
Martinez, K. ;
Zema, P. ;
Arias, A. ;
Perez, O. E. ;
Pilosof, A. M. R. .
JOURNAL OF FOOD ENGINEERING, 2012, 108 (03) :463-472
[2]  
Credence Research, 2022, HEMP OIL MARKET
[3]   Optimizing the potential bioactivity of isoflavones from soybeans via ultrasound pretreatment: Antioxidant potential and NF-κB activation [J].
Falcao, Heloisa Gabriel ;
Ramos Silva, Mariah Benine ;
de Camargo, Adriano Costa ;
Shahidi, Fereidoon ;
Franchin, Marcelo ;
Rosalen, Pedro Luiz ;
Alencar, Severino Matias ;
Kurozawa, Louise Emy ;
Ida, Elza Iouko .
JOURNAL OF FOOD BIOCHEMISTRY, 2019, 43 (11)
[4]   Analysis of the kinetics of folding of proteins and peptides using circular dichroism [J].
Greenfield, Norma J. .
NATURE PROTOCOLS, 2006, 1 (06) :2891-2899
[5]   Hempseed meal protein isolates prepared by different isolation techniques. Part I. physicochemical properties [J].
Hadnadev, Miroslav ;
Dapcevic-Hadnadev, Tamara ;
Lazaridou, Athina ;
Moschakis, Thomas ;
Michaelidou, Alexandra-M. ;
Popovic, Senka ;
Biliaderis, Costas G. .
FOOD HYDROCOLLOIDS, 2018, 79 :526-533
[6]   Effects of ultrasound on structural and physical properties of soy protein isolate (SPI) dispersions [J].
Hu, Hao ;
Wu, Jiahui ;
Li-Chan, Eunice C. Y. ;
Zhu, Le ;
Zhang, Fang ;
Xu, Xiaoyun ;
Fan, Gang ;
Wang, Lufeng ;
Huang, Xingjian ;
Pan, Siyi .
FOOD HYDROCOLLOIDS, 2013, 30 (02) :647-655
[7]   Effects of ultrasound on the structure and physical properties of black bean protein isolates [J].
Jiang, Lianzhou ;
Wang, Jing ;
Li, Yang ;
Wang, Zhongjiang ;
Liang, Jing ;
Wang, Rui ;
Chen, Yong ;
Ma, Wenjun ;
Qi, Baokun ;
Zhang, Min .
FOOD RESEARCH INTERNATIONAL, 2014, 62 :595-601
[8]   Modifying the physicochemical properties of pea protein by pH-shifting and ultrasound combined treatments [J].
Jiang, Shanshan ;
Ding, Junzhou ;
Andrade, Juan ;
Rababah, Taha M. ;
Almajwal, Ali ;
Abulmeaty, Mahmoud M. ;
Feng, Hao .
ULTRASONICS SONOCHEMISTRY, 2017, 38 :835-842
[9]   Modification of hemp seed protein isolate (Cannabis sativa L.) by high-intensity ultrasound treatment. Part 1: Functional properties [J].
Karabulut, Gulsah ;
Yemis, Oktay .
FOOD CHEMISTRY, 2022, 375
[10]   Effect of Ultrasound on Alkali Extraction Protein from Rice Dreg Flour [J].
Li, Kexin ;
Ma, Haile ;
Li, Shujun ;
Zhang, Cunsheng ;
Dai, Chunhua .
JOURNAL OF FOOD PROCESS ENGINEERING, 2017, 40 (02)
123