Case report: Whole genome sequence of Clostridium perfringens JUM001 causing acute emphysematous cholecystitis

被引：0

作者：

Tohya, Mari ^{[1
,2
]}

Otsuka, Tomohiro ^{[3
]}

Yoshimoto, Jiro ^{[3
]}

Ishizaki, Yoichi ^{[3
]}

Kirikae, Teruo ^{[1
,2
]}

Watanabe, Shin ^{[2
]}

机构：

[1] Juntendo Univ, Sch Med, Dept Microbiol, Tokyo, Japan

[2] Juntendo Univ, Sch Med, Dept Microbiome Res, Tokyo, Japan

[3] Juntendo Univ, Urayasu Hosp, Dept Digest & Gen Surg, Chiba, Japan

来源：

FRONTIERS IN MICROBIOLOGY | 2022年 / 13卷

关键词：

Clostridium perfringens; emphysematous cholecystitis; whole-genome sequencing; toxinotype A; alpha-toxin; pCP13-like family plasmid; pCW3-like family plasmid; CLOSTRIDIUM-PERFRINGENS; ENTEROTOXIN; ALIGNMENT; PLASMID;

D O I：

10.3389/fmicb.2022.1066880

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A strain of Clostridium perfringens was isolated from the bile sample of a patient with emphysematous cholecystitis who underwent a laparoscopic cholecystectomy, followed by treatment with meropenem and recovery. Metagenomic analysis of the bile sample showed that 99.73% of the bile microbiota consisted of C. perfringens, indicating that C. perfringens JUM001 was the causative pathogen of acute emphysematous cholecystitis in this patient. Complete genome sequencing showed that C. perfringens JUM001 contained a circular chromosome of 3,231,023 bp and two circular plasmids, pJUM001-1 of 49,289 bp and pJUM001-2 of 47,855 bp. JUM001 was found to possess a typing toxin gene, plc, but no other typing toxin genes, indicating that its toxinotype is type A. The plasmids pJUM001-1 and pJUM001-2 belonged to the pCP13-like and pCW3-like families of plasmids, respectively, which are characteristic conjugative and archetypical plasmids of C. perfringens. Phylogenetic analysis showed that JUM001 was closely related to C. perfringens strain JXNC-DD isolated from a dog in China. To our knowledge, this is the first report of whole-genome sequences of a clinical isolate of C. perfringens causing acute emphysematous cholecystitis.

引用

页数：8

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