Differences in oxidative stress in heavy metal resistant and sensitive clones of Salix viminalis

被引:79
作者
Landberg, T [1 ]
Greger, M [1 ]
机构
[1] Univ Stockholm, Dept Bot, S-10691 Stockholm, Sweden
关键词
heavy metal; Cd; Cu; Zn; Salix; stress; antioxidative compounds; salicylic acid;
D O I
10.1078/0176-1617-00504
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Many stress mechanisms, such as antioxidative mechanisms, are known to be activated by heavy metals. The aim of this work was to investigate whether oxidative stress and related mechanisms were expressed differently in plants with different resistances to heavy metals. Different clones of Safix viminalis with different resistances to Cd, Cu, and Zn were cultivated hydroponically in the presence of 7mumol/L Cd, 3 mumol/L Cu or 70 mumol/L Zn for 20 days. The clones were then compared with regard to the concentrations of free radicals, estimated by measuring thiobarbituric acid-reactive material (TBA-rm) and glutathione (GSH). The activity of the enzymes, aspartate peroxidase (APX), guaiacol peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT) was also analysed. Salicylic acid was also measured since it is known to be involved in antioxidative activities. The results showed that some differences could be observed between resistant and sensitive clones. The SOD activity was higher in untreated resistant clones compared with the sensitive ones. Under metal treatment, however, the SOD activity was similar. Furthermore, TBA-rm was higher in shoots of resistant clones compared to sensitive ones, while the opposite was found in roots.
引用
收藏
页码:69 / 75
页数:7
相关论文
共 28 条
[21]   TOLERANCE AND CO-TOLERANCE OF THE GRASS CHLORIS-BARBATA SW TO MERCURY, CADMIUM AND ZINC [J].
PATRA, J ;
LENKA, M ;
PANDA, BB .
NEW PHYTOLOGIST, 1994, 128 (01) :165-171
[22]   SYSTEMIC INDUCTION OF SALICYLIC-ACID ACCUMULATION IN CUCUMBER AFTER INOCULATION WITH PSEUDOMONAS-SYRINGAE PV SYRINGAE [J].
RASMUSSEN, JB ;
HAMMERSCHMIDT, R ;
ZOOK, MN .
PLANT PHYSIOLOGY, 1991, 97 (04) :1342-1347
[23]   PHYTOCHELATINS [J].
RAUSER, WE .
ANNUAL REVIEW OF BIOCHEMISTRY, 1990, 59 :61-86
[24]   TOXIC OXYGEN SPECIES AND PROTECTIVE SYSTEMS OF THE CHLOROPLAST [J].
SALIN, ML .
PHYSIOLOGIA PLANTARUM, 1988, 72 (03) :681-689
[25]   COPPER-MEDIATED LIPID-PEROXIDATION PROCESSES IN PHOTOSYNTHETIC MEMBRANES [J].
SANDMANN, G ;
BOGER, P .
PLANT PHYSIOLOGY, 1980, 66 (05) :797-800
[26]   Salicylic acid modulates glutathione metabolism in pea seedlings [J].
Srivastava, MK ;
Dwivedi, UN .
JOURNAL OF PLANT PHYSIOLOGY, 1998, 153 (3-4) :409-414
[27]   MEASUREMENT OF TOLERANCE TO EDAPHIC FACTORS BY MEANS OF ROOT-GROWTH [J].
WILKINS, DA .
NEW PHYTOLOGIST, 1978, 80 (03) :623-633
[28]  
YALPANI N, 1994, PLANTA, V193, P372, DOI 10.1007/BF00201815