Giant Vesicles Containing Superparamagnetic Iron Oxide as Biodegradable Cell-Tracking MRI Probes

被引：21

作者：

Toyota, Taro ^{[3
]}

Ohguri, Naoto ^{[3
]}

Maruyama, Kouichi ^{[2
]}

Fujinami, Masanori ^{[3
]}

Saga, Tsuneo ^{[1
]}

Aoki, Ichio ^{[1
]}

机构：

[1] Natl Inst Radiol Sci, Mol Imaging Ctr, Inage Ku, Chiba 2638555, Japan

[2] Natl Inst Radiol Sci, Res Ctr Radiat Protect, Inage Ku, Chiba 2638555, Japan

[3] Chiba Univ, Grad Sch Engn, Dept Appl Chem & Biotechnol, Inage Ku, Chiba 2638522, Japan

来源：

ANALYTICAL CHEMISTRY | 2012年 / 84卷 / 09期

关键词：

MAGNETICALLY LABELED CELLS; IN-VIVO; CONTRAST AGENTS; ORYZIAS-LATIPES; LIPOSOMES; NANOPARTICLES; MEDAKA; FEASIBILITY; STABILITY; PARTICLES;

D O I：

10.1021/ac2031354

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A major breakthrough in in vivo cellular imaging has been the clinical/preclinical use of magnetic resonance imaging (MRI) with contrast agent. Superparamagnetic iron oxide (SPIO) is a promising candidate for the development of smart MRI probes for cell-tracking. In the present study, we describe biodegradable probes made of giant vesicles (GVs; closed lipid membranes with diameters >1 mu m) that encapsulate SPIO for use as an MRI contrast agent. These SPIO-containing GVs (SPIO-GVs) exhibited excellent contrast enhancement in the single cell of medaka fish (Oryzias latipes) embryos immediately after their microinjection, and this enhancement disappeared when the GV membranes were destroyed. Our results demonstrate that SPIO-GVs are useful MRI probes for single cell-tracking that have minimum cytotoxicity and will greatly improve clinical/preclinical in vivo cellular imaging techniques.

引用

页码：3952 / 3957

页数：6

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