Development of an immunoassay for the derived-peptide of chromogranin A, Vasostatin-I (1-76): assessment of severity in patients with sepsis

被引:9
作者
Chung, Helene [1 ]
Corti, Angelo [2 ]
Crippa, Luca [2 ]
Schneider, Francis [3 ]
Metz-Boutigue, Marie-Helene [4 ]
Garnero, Patrick [1 ]
机构
[1] Cisbio Bioassays, F-30200 Codolet, France
[2] Ist Sci San Raffaele, Div Mol Oncol, I-20132 Milan, Italy
[3] Hop Univ Strasbourg, Hop Hautepierre, Serv Reanimat Med, Strasbourg, France
[4] Univ Strasbourg, Lab Biomat & Ingn Tissulaire, Strasbourg, France
关键词
Biomarker; chromogranin A; sepsis; vasostatin; HEART; BIOMARKERS; FRAGMENT; FAILURE;
D O I
10.3109/1354750X.2012.680610
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Context: Proteolytic fragments of chromogranin A (CgA) including the CgA 1-76 fragment (called vasostatin-I [VS-I]) could be a useful biomarker of sepsis, but there is no available immunoassay. Methods: A sandwich ELISA for VS-I was developed, and plasma VS-I was measured in 30 healthy controls and 60 critically ill patients with sepsis. Results: The ELISA showed intra-and inter-assay coefficients of variations (CVs) below 4 and 9%. Plasma VS-I was significantly increased compared with controls in patients with sepsis, severe sepsis, and sepsis shock (p < 0.0001). Receiver operating curve (ROC) analyses indicated that plasma VS-I was more sensitive and specific than plasma CgA to diagnose sepsis and to assess its severity. Conclusions: The measurements of plasma VS-I with this new ELISA may be useful for the clinical investigation of patients with sepsis.
引用
收藏
页码:430 / 434
页数:5
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