In-Cell NMR: Analysis of Protein-Small Molecule Interactions, Metabolic Processes, and Protein Phosphorylation

被引：11
作者：
Kumar, Amit [1 ,2 ,3 ]
Kuhn, Lars T. [1 ]
Balbach, Jochen [2 ,4 ]
机构：
[1] Univ Leeds, Sch Mol & Cellular Biol, Astbury Ctr Struct Mol Biol, Leeds LS2 9JT, W Yorkshire, England
[2] Martin Luther Univ Halle Wittenberg, Inst Phys, Biophys, D-06120 Halle, Germany
[3] Kings Coll London, Fac Lifesci & Med, Dept Diabet, London SE1 1UL, England
[4] Martin Luther Univ Halle Wittenberg, Ctr Struct & Dynam Proteins MZP, D-06120 Halle, Germany
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2019年 / 20卷 / 02期
关键词：
protein NMR; in-cell NMR; in-situ NMR; DNP; review; PARATHYROID-HORMONE PTH; LIVING ESCHERICHIA-COLI; PROLYL ISOMERASE; PARA-HYDROGEN; POSTTRANSLATIONAL MODIFICATIONS; MULTIDIMENSIONAL NMR; F-19; NMR; SPECTROSCOPY; DOMAIN; HYPERPOLARIZATION;
D O I：
10.3390/ijms20020378
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Nuclear magnetic resonance (NMR) spectroscopy enables the non-invasive observation of biochemical processes, in living cells, at comparably high spectral and temporal resolution. Preferably, means of increasing the detection limit of this powerful analytical method need to be applied when observing cellular processes under physiological conditions, due to the low sensitivity inherent to the technique. In this review, a brief introduction to in-cell NMR, protein-small molecule interactions, posttranslational phosphorylation, and hyperpolarization NMR methods, used for the study of metabolites in cellulo, are presented. Recent examples of method development in all three fields are conceptually highlighted, and an outlook into future perspectives of this emerging area of NMR research is given.
引用
收藏
页数：23
相关论文
共 130 条
[91]  
OVERHAUSER AW, 1953, PHYS REV, V92, P411, DOI 10.1103/PhysRev.92.411
[92]   Cell cycle: Reaching for a role for the Cks proteins [J].
Pines, J .
CURRENT BIOLOGY, 1996, 6 (11) :1399-1402
[93]   Protein kinase CK2: a challenge to canons [J].
Pinna, LA .
JOURNAL OF CELL SCIENCE, 2002, 115 (20) :3873-3878
[94]   NET NMR ALIGNMENT BY ADIABATIC TRANSPORT OF PARA-HYDROGEN ADDITION-PRODUCTS TO HIGH MAGNETIC-FIELD [J].
PRAVICA, MG ;
WEITEKAMP, DP .
CHEMICAL PHYSICS LETTERS, 1988, 145 (04) :255-258
[95]   Antimicrobial resistance: a global multifaceted phenomenon [J].
Prestinaci, Francesca ;
Pezzotti, Patrizio ;
Pantosti, Annalisa .
PATHOGENS AND GLOBAL HEALTH, 2015, 109 (07) :309-318
[96]   Probing the interaction of a quercetin bioconjugate with Bcl-2 in living human cancer cells with in-cell NMR spectroscopy [J].
Primikyri, Alexandra ;
Sayyad, Nisar ;
Quilici, Giacomo ;
Vrettos, Eirinaios I. ;
Lim, Kyungeun ;
Chi, Seung-Wook ;
Musco, Giovanna ;
Gerothanassis, Ioannis P. ;
Tzakos, Andreas G. .
FEBS LETTERS, 2018, 592 (20) :3367-3379
[97]  
RABBANI SA, 1984, J BIOL CHEM, V259, P2949
[98]   ParaHydrogen Induced Polarization of 13C carboxylate resonance in acetate and pyruvate [J].
Reineri, Francesca ;
Boi, Tommaso ;
Aime, Silvio .
NATURE COMMUNICATIONS, 2015, 6
[99]   New NMR tools for protein structure and function: Spin tags for dynamic nuclear polarization solid state NMR [J].
Rogawski, Rivkah ;
McDermott, Ann E. .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2017, 628 :102-113
[100]   An N-terminal molecular form of parathyroid hormone (PTH) distinct from hPTH(1-84) is overproduced in parathyroid carcinoma [J].
Rubin, Mishaela R. ;
Silverberg, Shonni J. ;
D'Amour, Pierre ;
Brossard, Jean-Hugues ;
Rousseau, Louise ;
Sliney, James, Jr. ;
Cantor, Tom ;
Bilezikian, John P. .
CLINICAL CHEMISTRY, 2007, 53 (08) :1470-1476
45678910111213