miRNA-449a is downregulated in osteosarcoma and promotes cell apoptosis by targeting BCL2

被引：47

作者：

Chen, Jie ^{[1
]}

Zhou, Jinsong ^{[1
,2
]}

Chen, Xin ^{[1
]}

Yang, Baohui ^{[1
]}

Wang, Dong ^{[1
]}

Yang, Pinglin ^{[1
]}

He, Xijing ^{[1
]}

Li, Haopeng ^{[1
]}

机构：

[1] Xi An Jiao Tong Univ, Sch Med, Affiliated Hosp 2, Dept Orthopaed Surg, Xian 710004, Shaanxi, Peoples R China

[2] Xian 5 Hosp, Dept Orthopaed Surg, Xian 710004, Shaanxi, Peoples R China

来源：

TUMOR BIOLOGY | 2015年 / 36卷 / 10期

关键词：

miR-449a; Osteosarcoma; Tumor suppressor; Apoptosis; BCL2; EXPRESSION; INVASION; PROLIFERATION; CANCER; GROWTH;

D O I：

10.1007/s13277-015-3568-y

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Accumulating evidence reveals that miR-449a is expressed at a low level in several tumors and cancer cell lines, and acts as a tumor suppressor in several cancers. However, its role in osteosarcoma (OS) is not well understood. In the present study, we found that miR-449a was significantly downregulated in both OS tissues and cell lines. Furthermore, low expression level of miR-449a was correlated with advanced tumor stage, metastasis, and predicted a poor overall survival in OS patients. Additionally, restoration of miR-449a in OS cell lines U2OS and Saos-2 reduced cell viability, promoted cell apoptosis in vitro, and suppressed tumorigenicity in vivo. Moreover, BCL2, an antiapoptotic molecule, was identified to be a direct target of miR-449a, and the proapoptotic function of miR-449a was mainly through targeting BCL2 expression. Taken together, our results demonstrated a tumor-suppressive role of miR-449a in OS progression and suggested a potential therapeutic target for OS.

引用

页码：8221 / 8229

页数：9

共 27 条

[1]

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