Photosensitizer-Loaded Magnetic Nanoemulsion for Use in Synergic Photodynamic and Magnetohyperthermia Therapies of Neoplastic Cells

被引：34

作者：

Primo, Fernando L. ^{[2
]}

Rodrigues, Marcilene M. A. ^{[1
]}

Simioni, Andreza Ribeiro ^{[1
]}

Lacava, Zulmira G. M. ^{[3
]}

Morais, Paulo C. ^{[4
]}

Tedesco, Antonio C. ^{[1
]}

机构：

[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Pret, Lab Fotobiol & Fotomed, Dept Quim, BR-14040901 Ribeirao Preto, SP, Brazil

[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, BR-14040903 Ribeirao Preto, SP, Brazil

[3] Univ Brasilia, Inst Ciencias Biol, Dept Genet & Morfol, BR-70910900 Brasilia, DF, Brazil

[4] Univ Brasilia, Inst Fis, BR-70910900 Brasilia, DF, Brazil

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2008年 / 8卷 / 11期

基金：

巴西圣保罗研究基金会;

关键词：

Nanotechnology; Magnetic Nanoemulsion; Photodynamic Therapy; J774-A1; Cells; Zinc Phthalocyanine; Hyperthermia;

D O I：

10.1166/jnn.2008.476

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this study a magnetic nanoemulsion (MNE) was developed from a mixture of two components, namely biodegradable surfactants and biocompatible citrate-coated cobalt ferrite-based magnetic fluid, for entrapment of Zn(II)-Phthalocyanine (ZnPc), the latter a classical photosensitizer (PS) species used in photodynamic therapy (PDT) procedures. The sample's stability was evaluated as a function of time using photocorrelation spectroscopy (PCS) for determination of the average hydrodynamic diameter, diameter dispersion and zeta potential. The ZnPc-loaded magneto nanoemulstion (ZnPc/MNE) formulation was evaluated in vitro assays to access the phototoxicity and the effect of application of AC magnetic fields (magnetohyperthermia damage) after incubation with J774-A1 macrophages cells. Darkness toxicity, phototoxicity and AC magnetic field exposures revealed an enhancement response for combined photodynamic and magnetohyperthermia (MHT) processes, indicating the presence of the synergic effect.

引用

页码：5873 / 5877

页数：5

共 25 条

[1]

COUVREUR P, 1992, J CONTROL RELEASE, V19, P259, DOI 10.1016/0168-3659(92)90081-2

[2]

COUVREUR P, 1995, EUR J PHARM BIOPHARM, V41, P2

[3] Paclitaxel-loaded PLGA nanoparticles: preparation, physicochemical characterization and in vitro anti-tumoral activity [J].