Ion Chromatographic Fingerprinting of STC-1 Cellular Response for Taste Sensing

被引：7

作者：

Zabadaj, Marcin ^{[1
]}

Szuplewska, Aleksandra ^{[1
]}

Balcerzak, Maria ^{[2
]}

Chudy, Michal ^{[1
]}

Ciosek-Skibinska, Patrycja ^{[1
]}

机构：

[1] Warsaw Univ Technol, Chair Med Biotechnol, Fac Chem, Noakowskiego 3, PL-00664 Warsaw, Poland

[2] Warsaw Univ Technol, Fac Chem, Noakowskiego 3, PL-00664 Warsaw, Poland

来源：

SENSORS | 2019年 / 19卷 / 05期

关键词：

taste sensing; bioelectronic tongue; STC-1; cells; ion chromatography; chromatographic fingerprints; PLS-DA; BIOELECTRONIC TONGUE; ELECTRONIC TONGUE; RECEPTOR; BIOSENSOR; SWEET; CELLS; PERFORMANCE; SENSORS; UMAMI;

D O I：

10.3390/s19051062

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Taste sensing is of great importance in both the pharmaceutical and foodstuff industries, and is currently mainly based on human sensory evaluation. Many approaches based on chemical sensors have been proposed, leading to the development of various electronic tongue systems. However, this approach is limited by the applied recognition methods, which do not consider natural receptors. Biorecognition elements such as taste receptor proteins or whole cells can be involved in the development of taste sensing biosensors usually equipped with various electrochemical transducers. Here, we propose a new approach: intestinal secretin tumor cell line (STC-1) chemosensory cells were applied for taste recognition, and their taste-specific cellular response was decoded from ion chromatographic fingerprints with the use of multivariate data processing by partial least squares discriminant analysis (PLS-DA). This approach could be useful for the development of various non-invasive taste sensing assays, as well as for studying taste transduction mechanisms in vitro.

引用

页数：10

共 31 条

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