Isolation and characterization of microsatellites in Dendrobium crystallinum and application to germplasm identification

被引：4

作者：

Ding, Ge ^{[1
]}

Zhang, Daizhen ^{[2
]}

Ding, Xiaoyu ^{[3
]}

机构：

[1] Yancheng Inst Technol, Chem & Biol Engn Coll, Yancheng, Peoples R China

[2] Yancheng Normal Univ, Jiangsu Prov Key Lab Coastal Wetland Bioresources, Yancheng, Peoples R China

[3] Nanjing Normal Univ, Coll Life Sci, Jiangsu Prov Key Lab Biodivers & Biotechnol, Nanjing, Jiangsu, Peoples R China

来源：

CONSERVATION GENETICS RESOURCES | 2015年 / 7卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Dendrobium crystallinum; Microsatellite; SSR; Genetic diversity;

D O I：

10.1007/s12686-015-0449-z

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Dendrobium crystallinum is an endangered species with good restorative and medicinal functions. To investigate the genetic diversity and structure of this species, we isolated and characterized 20 microsatellite loci derived from two microsatellite-enriched libraries. Thirty individuals from a population in Tiane were used to evaluate the genetic characteristics of the microsatellites. The numbers of alleles per locus ranged from 3 to 10 with a mean of 4.9. The observed and expected heterozygosities ranged from 0.164 to 0.602 and from 0.179 to 0.653, respectively. Transferability tests between the congeneric species and the identifications of the plantlets in the same population were successful. The 20 microsatellite loci characterized in this study will be useful for future population structure, genetic diversity and conservation genetic investigations.

引用

页码：735 / 737

页数：3

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