Structural insights into P-glycoprotein (ABCB1) by small angle X-ray scattering and electron crystallography

被引：19
作者：
McDevitt, Christopher A. [1 ]
Shintre, Chitra A. [2 ]
Grossmann, J. Guenter [3 ]
Pollock, Naomi L. [1 ]
Prince, Stephen M. [2 ]
Callaghan, Richard [1 ]
Ford, Robert C. [2 ]
机构：
[1] Univ Oxford, John Radcliffe Hosp, Nuffield Dept Clin Lab Sci, Oxford OX3 9DU, England
[2] Univ Manchester, Fac Life Sci, Manchester Interdisciplinary Bioctr, Manchester M1 7DN, Lancs, England
[3] Univ Liverpool, Sch Biol Sci, Mol Biophys Grp, Liverpool L69 72B, Merseyside, England
来源：
FEBS LETTERS | 2008年 / 582卷 / 19期
基金：
英国医学研究理事会;
关键词：
multidrug resistance; membrane protein; protein structure; electron crystallography; SAXS; ABCB1;
D O I：
10.1016/j.febslet.2008.07.022
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
P-glycoprotein (ABCB1) is an ATP-binding cassette protein that is associated with the acquisition of multi-drug resistance in cancer and the failure of chemotherapy in humans. Structural insights into this protein are described using a combination of small angle X-ray scattering data and cryo-electron crystallography data. We have compared the structures with bacterial homologues, and discuss the development of homology models for P-glycoprotein based on the bacterial Sav1866 structure. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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收藏
页码：2950 / 2956
页数：7
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共 47 条
  • [1] STRUCTURAL HOMOLOGY OF REACTION CENTERS FROM RHODOPSEUDOMONAS-SPHAEROIDES AND RHODOPSEUDOMONAS-VIRIDIS AS DETERMINED BY X-RAY-DIFFRACTION
    ALLEN, JP
    FEHER, G
    YEATES, TO
    REES, DC
    DEISENHOFER, J
    MICHEL, H
    HUBER, R
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (22) : 8589 - 8593
  • [2] 3-DIMENSIONAL STRUCTURE DETERMINATION BY ELECTRON-MICROSCOPY OF TWO-DIMENSIONAL CRYSTALS
    AMOS, LA
    HENDERSON, R
    UNWIN, PNT
    [J]. PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1983, 39 (03) : 183 - 231
  • [3] Nanostructure of fibrillin-1 reveals compact conformation of EGF arrays and mechanism for extensibility
    Baldock, Clair
    Siegler, Veronique
    Bax, Daniel V.
    Cain, Stuart A.
    Mellody, Kieran T.
    Marson, Andrew
    Haston, J. Louise
    Berry, Richard
    Wang, Ming-Chuan
    Grossmann, J. Gunter
    Roessle, Manfred
    Kielty, Cay M.
    Wess, Tim J.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (32) : 11922 - 11927
  • [4] The translocation mechanism of P-glycoprotein
    Callaghan, R
    Ford, RC
    Kerr, ID
    [J]. FEBS LETTERS, 2006, 580 (04) : 1056 - 1063
  • [5] Structure of the multidrug ABC transporter Sav1866 from Staphylococcus aureus in complex with AMP-PNP
    Dawson, Roger J. P.
    Locher, Kaspar P.
    [J]. FEBS LETTERS, 2007, 581 (05) : 935 - 938
  • [6] Structure of a bacterial multidrug ABC transporter
    Dawson, Roger J. P.
    Locher, Kaspar P.
    [J]. NATURE, 2006, 443 (7108) : 180 - 185
  • [7] Electron crystallography of biomolecules: mysterious membranes and missing cones
    Ford, Robert C.
    Holzenburg, Andreas
    [J]. TRENDS IN BIOCHEMICAL SCIENCES, 2008, 33 (01) : 38 - 43
  • [8] SPIDER and WEB: Processing and visualization of images in 3D electron microscopy and related fields
    Frank, J
    Radermacher, M
    Penczek, P
    Zhu, J
    Li, YH
    Ladjadj, M
    Leith, A
    [J]. JOURNAL OF STRUCTURAL BIOLOGY, 1996, 116 (01) : 190 - 199
  • [9] Structure of the ABC ATPase domain of human TAP1, the transporter associated with antigen processing
    Gaudet, R
    Wiley, DC
    [J]. EMBO JOURNAL, 2001, 20 (17) : 4964 - 4972
  • [10] Visualizing density maps with UCSF Chimera
    Goddard, Thomas D.
    Huang, Conrad C.
    Ferrin, Thomas E.
    [J]. JOURNAL OF STRUCTURAL BIOLOGY, 2007, 157 (01) : 281 - 287
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