Injectable thermogelling chitosan for the local delivery of bone morphogenetic protein

被引：17

作者：

McLaughlin, Shaun W. ^{[2
]}

Cui, Zhanwu ^{[1
]}

Starnes, Trevor ^{[3
]}

Laurencin, Cato T. ^{[1
,4
]}

Kan, Ho-Man ^{[1
]}

Wu, Qian ^{[5
]}

Nair, Lakshmi S. ^{[1
,4
]}

机构：

[1] Univ Connecticut, Ctr Hlth, Dept Orthopead Surg, Inst Regenerat Engn, Farmington, CT 06030 USA

[2] Univ Connecticut, Ctr Hlth, Sch Med, Farmington, CT 06030 USA

[3] Univ Virginia, Sch Med, Charlottesville, VA 22903 USA

[4] Univ Connecticut, Inst Mat Sci, Dept Chem Mat & Biomol Engn, Storrs, CT 06269 USA

[5] Univ Connecticut, Dept Pathol & Lab Med, Ctr Hlth, Farmington, CT 06030 USA

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 2012年 / 23卷 / 09期

关键词：

CONTROLLED-RELEASE; GRAFTED CHITOSAN; SCAFFOLD; REPAIR; BIOCOMPATIBILITY; IMPLANTATION; NEUTROPHILS; PHOSPHATE; COPOLYMER; CELLS;

D O I：

10.1007/s10856-012-4677-4

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The aim of the present study was to evaluate the in vivo biocompatibility of injectable thermo gelling chitosan-ammonium hydrogen phosphate solution (chitosan-AHP) and its efficacy to deliver recombinant human bone morphogenetic protein-2 (rhBMP-2) in a bioactive form. The thermogel showed a typical foreign body response upon subcutaneous implantation surrounded by a fibrous capsule. Even at 4 and 8 weeks post implantation, significant neutrophil infiltration was observed within the gel. Chitosan-AHP gel retained most of the loaded rhBMP-2 after a small initial release. The bioactivity of the released protein was demonstrated in vitro by the increase in alkaline phosphatase activity of mouse pre osteoblast cells (MC3T3-E1). Histological and micro-computed tomography (mu CT) evaluation showed evidence of ectopic bone formation upon 4 mu g/mL rhBMP-2 loaded chitosan-AHP injection. The study demonstrated a neutrophil mediated local tissue response to chitosan-AHP gel and its ability to encapsulate and maintain the bioactivity of rhBMP-2.

引用

页码：2141 / 2149

页数：9

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