Optimization of treatment with recombinant FGF-2 for proliferation and differentiation of human dental stem cells, mesenchymal stem cells, and osteoblasts

被引：22

作者：

Lee, Tae-Hyung ^{[1
,2
]}

Kim, Won-Tae ^{[3
]}

Ryu, Chun Jeih ^{[3
]}

Jang, Young-Joo ^{[1
,2
]}

机构：

[1] Dankook Univ, Dept Nanobiomed Sci, Cheonan 330714, South Korea

[2] Dankook Univ, PLUS Global Res Ctr Regenerat Med BK21, Cheonan 330714, South Korea

[3] Sejong Univ, Dept Biosci & Biotechnol, Seoul 143747, South Korea

来源：

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 2015年 / 93卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

bFGF; human dental pulp stem cells; mesenchymal stem cells; cell proliferation/differentiation; mineralization; FIBROBLAST-GROWTH-FACTOR; CHONDROGENIC DIFFERENTIATION; IN-VITRO; FACTOR-2; EXPRESSION; TISSUE; IMMUNOLOCALIZATION; ANGIOGENESIS; POTENTIALS; MAINTAIN;

D O I：

10.1139/bcb-2014-0140

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Basic fibroblast growth factor (bFGF or FGF-2) is widely used to modulate the proliferation and differentiation of certain cell types. An expression and purification system for recombinant human FGF-2 in Escherichia coli was established for the purpose of securing a continuous supply of this protein. The purified recombinant FGF-2 significantly increased the population of human embryonic stem cells. The optimal concentrations of FGF-2 for cell proliferative induction in various adult stem cells including human dental pulp stem cells, full term human periodontal ligament stem cells, human gingival fibroblasts, mesenchymal stem cells, and osteogenic oseosarcoma were established in a dose-dependent manner. When cells were treated with recombinant FGF-2 for 6 days before osteogenic induction, the mRNA expression of the bone markers was upregulated in cells originated from human dental pulp tissue, indicating that pretreatment with FGF-2 during culture increase stem cell/progenitor population and osteogenic potential.

引用

页码：298 / 305

页数：8

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