Specificity of IgE antibodies from patients allergic to goat's milk and tolerant to cow's milk determined with plasmin-derived peptides of bovine and caprine β-caseins

被引:11
作者
Bernard, Herve [1 ]
Ah-Leung, Sandrine
Tilleul, Sylvain
Drumare, Marie-Francoise
Paty, Evelyne [2 ]
Bidat, Etienne [3 ]
Wal, Jean-Michel
Hazebrouck, Stephane
机构
[1] CEA Saclay, Lab INRA Immunoallergie Alimentaire, iBiTec S, INRA,UR 496,Unit Immunoallergie Alimentaire,CEA,S, F-91191 Gif Sur Yvette, France
[2] Univ Paris 05, Hop Necker Enfants Malad, Assistance Publ Hop Paris, Paris, France
[3] Hop Ambroise Pare, Assistance Publ Hop Paris, Serv Pediat, Boulogne, France
关键词
Allergy; Casein; Epitope; Goat's milk; IgE; BINDING EPITOPES; CLINICAL-COURSE; FOOD ALLERGY; CHEESE; CHILDREN; IDENTIFICATION; ALLERGENICITY; PREVALENCE; DELETION;
D O I
10.1002/mnfr.201200229
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Scope Despite a sequence homology of 90% between bovine and caprine beta-caseins (CN), IgE antibodies from patients allergic to goat's milk (GM), but tolerant to cow's milk (CM), recognize caprine beta-CN without cross-reacting with bovine beta-CN. We investigated this lack of cross-reactivity by evaluating the IgE-reactivity toward peptides isolated from plasmin hydolysates of bovine and caprine beta-CN. Methods and results The IgE-binding capacity of plasmin-derived peptides was evaluated with sera from 10 CM-allergic patients and 12 GM-allergic/CM-tolerant patients. In CM-allergic patients, IgE reactivity of caprine fragments (f29-107) and (f108-207), but not (f1-28), was similar to that of the bovine counterparts. In contrast, all bovine fragments were poorly recognized by IgE antibodies from GM-allergic/CM-tolerant patients. The peptide (f29-107) was generally the most immunoreactive fragment of caprine beta-CN. By using synthetic peptides, the immunodominant IgE-binding epitope recognized by most GM-allergic/CM-tolerant patients was located in the caprine domain 4979. Conclusion The restricted specificity of the IgE response toward the caprine beta-CN in GM-allergic/CM-tolerant patients is mainly directed against the domain 4979, which differs from its bovine counterpart by only three amino acid substitutions.
引用
收藏
页码:1532 / 1540
页数:9
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