"Solvent/non-solvent" treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds

被引：10
作者：
Goreninskii, S. I. [1 ,2 ]
Guliaev, R. O. [1 ]
Stankevich, K. S. [1 ,2 ]
Danilenko, N. V. [1 ]
Bolbasov, E. N. [2 ]
Golovkin, A. S. [3 ]
Mishanin, A. I. [3 ]
Filimonov, V. D. [1 ]
Tverdokhlebov, S. I. [2 ]
机构：
[1] Tomsk Polytech Univ, Natl Res, NM Kizhner Res & Educ Ctr, Tomsk, Russia
[2] Tomsk Polytech Univ, Natl Res, BP Veinberg Res & Educ Ctr, Tomsk, Russia
[3] Almazov Natl Med Res Ctr, St Petersburg, Russia
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2019年 / 177卷
关键词：
Polylactic add; Gelatin; Electrospun scaffolds; Surface modification; STEM-CELLS; DIFFERENTIATION; NANOFIBERS; FIBERS;
D O I：
10.1016/j.colsurfb.2019.01.060
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120 degrees to 0 degrees, enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 +/- 0.01 mg per cm(3) of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.
引用
收藏
页码：137 / 140
页数：4
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