"Solvent/non-solvent" treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds

被引：10

作者：

Goreninskii, S. I. ^{[1
,2
]}

Guliaev, R. O. ^{[1
]}

Stankevich, K. S. ^{[1
,2
]}

Danilenko, N. V. ^{[1
]}

Bolbasov, E. N. ^{[2
]}

Golovkin, A. S. ^{[3
]}

Mishanin, A. I. ^{[3
]}

Filimonov, V. D. ^{[1
]}

Tverdokhlebov, S. I. ^{[2
]}

机构：

[1] Tomsk Polytech Univ, Natl Res, NM Kizhner Res & Educ Ctr, Tomsk, Russia

[2] Tomsk Polytech Univ, Natl Res, BP Veinberg Res & Educ Ctr, Tomsk, Russia

[3] Almazov Natl Med Res Ctr, St Petersburg, Russia

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2019年 / 177卷

关键词：

Polylactic add; Gelatin; Electrospun scaffolds; Surface modification; STEM-CELLS; DIFFERENTIATION; NANOFIBERS; FIBERS;

D O I：

10.1016/j.colsurfb.2019.01.060

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120 degrees to 0 degrees, enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 +/- 0.01 mg per cm(3) of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.

引用

页码：137 / 140

页数：4

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