A plant peptide encoded by CLV3 identified by in situ MALDI-TOF MS analysis

被引：374

作者：

Kondo, Tatsuhiko

Sawa, Shinichiro ^{[1
]}

Kinoshita, Atsuko

Mizuno, Satoko

Kakimoto, Tatsuo

Fukuda, Hiroo

Sakagami, Youji

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Chikusa Ku, Nagoya, Aichi 4648601, Japan

[2] Univ Tokyo, Grad Sch Sci, Tokyo 1130033, Japan

[3] Osaka Univ, Grad Sch Sci, Toyonaka, Osaka 5600043, Japan

来源：

SCIENCE | 2006年 / 313卷 / 5788期

关键词：

D O I：

10.1126/science.1128439

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Arabidopsis CLAVATA3 (CLV3) gene encodes a stem cell - specific protein presumed to be a precursor of a secreted peptide hormone. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) applied to in situ Arabidopsis tissues determined the structure of a modified 12 - amino acid peptide (MCLV3), which was derived from a conserved motif in the CLV3 sequence. Synthetic MCLV3 induced shoot and root meristem consumption as cells differentiated into other organs, displaying the typical phenotype of transgenic plants overexpressing CLV3. These results suggest that the functional peptide of CLV3 is MCLV3.

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页码：845 / 848

页数：4

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