Biocatalytic properties of Baeyer-Villiger monooxygenases in aqueous-organic media

被引：65
作者：
de Gonzalo, G
Ottolina, G
Zambianchi, F
Fraaije, MW
Carrea, G
机构：
[1] CNR, Ist Chim Riconoscimento Mol, I-20131 Milan, Italy
[2] Univ Groningen, Biochem Lab, Groningen Biomol Sci, NL-9747 AG Groningen, Netherlands
[3] Univ Groningen, Inst Biotechnol, NL-9747 AG Groningen, Netherlands
来源：
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2006年 / 39卷 / 1-4期
关键词：
Baeyer-Villiger monooxygenases; aqueous-organic media; organic sulfides; chiral compounds; enzyme stability;
D O I：
10.1016/j.molcatb.2006.01.010
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The biocatalytic properties of three Baeyer-Villiger monooxygenases (phenylacetone monooxygenase, 4-hydroxyacetophenone monooxygenase and ethionamide monooxygenase) in a variety of aqueous-organic media were studied using organic sulfides as substrates. The influence of the nature and the concentration of the solvents, as well as of the substrates, on the activity and enantioselectivity of the enzymes was investigated in detail. Solvents were found to decrease, to a different extent, enzyme activity. High increases of enantioselectivity and also reversal of enantiopreference were observed depending on the enzyme and on the nature of the solvent and the substrate employed. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：91 / 97
页数：7
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