共 81 条
Mapping Condition-Dependent Regulation of Lipid Metabolism in Saccharomyces cerevisiae
被引:15
作者:
Jewett, Michael C.
[1
,3
,4
]
Workman, Christopher T.
[2
]
Nookaew, Intawat
[1
,5
,6
]
Pizarro, Francisco A.
[1
,7
]
Agosin, Eduardo
[1
,7
]
Hellgren, Lars I.
[2
]
Nielsen, Jens
[1
,5
]
机构:
[1] Tech Univ Denmark, Ctr Microbial Biotechnol, DTU Syst Biol, DK-2800 Lyngby, Denmark
[2] Tech Univ Denmark, Ctr Biol Sequence Anal, DTU Syst Biol, DK-2800 Lyngby, Denmark
[3] Northwestern Univ, Dept Chem & Biol Engn, Evanston, IL 60208 USA
[4] Northwestern Univ, Chem Life Proc Inst, Evanston, IL 60208 USA
[5] Chalmers Univ Technol, Dept Chem & Biol Engn, SE-41296 Gothenburg, Sweden
[6] King Mongkuts Univ Technol Thonburi, Fac Engn, Bangkok 10140, Thailand
[7] Pontificia Univ Catolica Chile, Sch Engn, Dept Chem & Bioproc Engn, Santiago 8320000, Chile
来源:
G3-GENES GENOMES GENETICS
|
2013年
/
3卷
/
11期
基金:
美国国家卫生研究院;
美国国家科学基金会;
关键词:
integrated systems biology;
lipid metabolism;
regulation;
metabolome;
omics;
FATTY-ACID;
TRANSCRIPTIONAL REGULATION;
PHOSPHOLIPID-SYNTHESIS;
CHEMOSTAT CULTURES;
STEROL SYNTHESIS;
YEAST;
INTEGRATION;
IDENTIFICATION;
BIOSYNTHESIS;
ORGANIZATION;
D O I:
10.1534/g3.113.006601
中图分类号:
Q3 [遗传学];
学科分类号:
071007 ;
090102 ;
摘要:
Lipids play a central role in cellular function as constituents of membranes, as signaling molecules, and as storage materials. Although much is known about the role of lipids in regulating specific steps of metabolism, comprehensive studies integrating genome-wide expression data, metabolite levels, and lipid levels are currently lacking. Here, we map condition-dependent regulation controlling lipid metabolism in Saccharomyces cerevisiae by measuring 5636 mRNAs, 50 metabolites, 97 lipids, and 57 C-13-reaction fluxes in yeast using a three-factor full-factorial design. Correlation analysis across eight environmental conditions revealed 2279 gene expression level-metabolite/lipid relationships that characterize the extent of transcriptional regulation in lipid metabolism relative to major metabolic hubs within the cell. To query this network, we developed integrative methods for correlation of multi-omics datasets that elucidate global regulatory signatures. Our data highlight many characterized regulators of lipid metabolism and reveal that sterols are regulated more at the transcriptional level than are amino acids. Beyond providing insights into the systems-level organization of lipid metabolism, we anticipate that our dataset and approach can join an emerging number of studies to be widely used for interrogating cellular systems through the combination of mathematical modeling and experimental biology.
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页码:1979 / 1995
页数:17
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