Assessment of reinforced poly(ethylene glycol) chitosan hydrogels as dressings in a mouse skin wound defect model

被引:82
作者
Chen, Szu-Hsien [1 ]
Tsao, Ching-Ting [1 ,2 ]
Chang, Chih-Hao [3 ,7 ]
Lai, Yi-Ting [4 ]
Wu, Ming-Fung [5 ]
Chuang, Ching-Nan [1 ]
Chou, Hung-Chia [4 ]
Wang, Chih-Kuang [6 ]
Hsieh, Kuo-Haung [1 ]
机构
[1] Natl Taiwan Univ, Coll Engn, Inst Polymer Sci & Engn, Taipei 10617, Taiwan
[2] Chang Gung Univ, Dept Anat, Epithelial Biol Lab, Transgen Mice Core Lab, Tao Yuan 33302, Taiwan
[3] Natl Taiwan Univ Hosp, Dept Orthoped, Taipei, Taiwan
[4] Natl Taiwan Univ, Coll Engn, Dept Chem Engn, Taipei 10617, Taiwan
[5] Natl Taiwan Univ, Coll Med, Anim Med Ctr, Taipei 10018, Taiwan
[6] Kaohsiung Med Univ, Dept Med & Appl Chem, Kaohsiung 80708, Taiwan
[7] Natl Taiwan Univ, Coll Med, Taipei 10018, Taiwan
来源
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2013年 / 33卷 / 05期
关键词
Chitosan; PEG; Mice; Hydrogel; Wound dressing; DRUG-DELIVERY SYSTEMS; FILMS;
D O I
10.1016/j.msec.2013.02.031
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Wound dressings of chitosan are biocompatible, biodegradable, antibacterial and hemostatic biomaterials. However, applications for chitosan are limited due to its poor mechanical properties. Here, we conducted an in vivo mouse angiogenesis study on reinforced poly(ethylene glycol) (PEG)-chitosan (RPC) hydrogels. RPC hydrogels were formed by cross-linking chitosan with PEGs of different molecular weights at various PEG to chitosan ratios in our previous paper. These dressings can keep the wound moist, had good gas exchange capacity, and was capable of absorbing or removing the wound exudate. We examined the ability of these RPC hydrogels and neat chitosan to heal small cuts and full-thickness skin defects on the backs of male Balb/c mice. Histological examination revealed that chitosan suppressed the infiltration of inflammatory cells and accelerated fibroblast proliferation, while PEG enhanced epithelial migration. The RPC hydrogels promoted wound healing in the small cuts and full layer wounds. The optimal RPC hydrogel had a swelling ratio of 100% and a water vapor transmission rate (WVTR) of about 2000 g/m(2)/day. In addition, they possess good mechanical property and appropriate degradation rates. Thus, the optimal RPC hydrogel formulation functioned effectively as a wound dressing and promoted wound healing. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:2584 / 2594
页数:11
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