Highly sensitive fluorescent protein FRET detection using optofluidic lasers

被引：101

作者：

Chen, Qiushu ^{[1
]}

Zhang, Xingwang ^{[1
,2
]}

Sun, Yuze ^{[1
]}

Ritt, Michael ^{[3
]}

Sivaramakrishnan, Sivaraj ^{[1
,3
]}

Fan, Xudong ^{[1
]}

机构：

[1] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA

[2] Fudan Univ, Dept Opt Sci & Engn, Minist Educ, Key Lab Micro & Nano Photon Struct, Shanghai 200433, Peoples R China

[3] Univ Michigan, Dept Cell & Dev Biol, Ann Arbor, MI 48109 USA

来源：

LAB ON A CHIP | 2013年 / 13卷 / 14期

基金：

中国国家自然科学基金; 美国国家科学基金会;

关键词：

DNA; SCAFFOLDS;

D O I：

10.1039/c3lc50207d

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We achieved optofluidic protein lasing using genetically encoded fluorescent protein FRET pairs linked by length-tunable peptides. Up to 25-fold reduction in the donor laser emission was observed when the donor and the acceptor were brought to close proximity, as compared to only 17% reduction in the donor emission using the conventional FRET detection. Our work opens a door to a broad range of applications in studying protein-protein interactions and protein-drug interactions.

引用

页码：2679 / 2681

页数：3

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