The GEN.S: a fortuitous finding of a routine screening test for hereditary spherocytosis

被引：23

作者：

Chiron, M ^{[1
]}

Cynober, T ^{[1
]}

Mielot, F ^{[1
]}

Tchernia, G ^{[1
]}

Croisille, L ^{[1
]}

机构：

[1] Univ Paris 11, Fac Med, Hop Bicetre, Hematol Lab, F-94270 Le Kremlin Bicetre, France

来源：

HEMATOLOGY AND CELL THERAPY | 1999年 / 41卷 / 03期

关键词：

reticulocytes; MCV; spherocytes; hereditary spherocytosis;

D O I：

10.1007/s00282-999-0113-8

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

As part of the evaluation of the GEN.S (Coulter(R)), we compared the Mean Corpuscular Volume (MCV) to the Mean Spherized Corpuscular Volume (MSCV) assessed during the reticulocyte count procedure under hypo-osmotic conditions. A sub-group of patients with hereditary spherocytosis (HS) was singled out: in all of them, the MSCV became smaller than the MCV. As the cell volume normally increases in red cells derived from other patients in the same conditions, we decided to further study the reason for this particular behaviour of HS red cells. Whereas normal red cells are able to undergo an osmotic expansion, the spherocytes reach a critical osmotic volume leading to cell fragmentation consistent with the decrease of MSCV. This fortuitous finding is likely to be a reliable improvement for the routine screening of HS.

引用

页码：113 / 116

页数：4