Ribosome-Inactivating Proteins: From Plant Defense to Tumor Attack

被引：116

作者：

de Virgilio, Maddalena ^{[2
]}

Lombardi, Alessio ^{[1
]}

Caliandro, Rocco ^{[3
]}

Fabbrini, Maria Serena ^{[1
]}

机构：

[1] CNR, Ist Biol & Biotecnol Agr, Milan, Italy

[2] CNR, Ist Genet Vegetale, Bari, Italy

[3] CNR, Ist Cristallog, Bari, Italy

来源：

TOXINS | 2010年 / 2卷 / 11期

关键词：

ribosome-inactivating proteins; cancer immunotherapy; ITs; vascular leak syndrome; recombinant expression; fusion toxins; single chain antibody variable fragments; Pichia pastoris; RICIN-A-CHAIN; POKEWEED ANTIVIRAL PROTEIN; PLASMINOGEN-ACTIVATOR RECEPTOR; RNA N-GLYCOSIDASE; B-CELL LYMPHOMA; X-RAY-STRUCTURE; PHASE-I TRIAL; ENDOPLASMIC-RETICULUM; FUSION PROTEIN; MONOCLONAL-ANTIBODY;

D O I：

10.3390/toxins2112699

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Ribosome-inactivating proteins (RIPs) are EC3.2.32.22 N-glycosidases that recognize a universally conserved stem-loop structure in 23S/25S/28S rRNA, depurinating a single adenine (A4324 in rat) and irreversibly blocking protein translation, leading finally to cell death of intoxicated mammalian cells. Ricin, the plant RIP prototype that comprises a catalytic A subunit linked to a galactose-binding lectin B subunit to allow cell surface binding and toxin entry in most mammalian cells, shows a potency in the picomolar range. The most promising way to exploit plant RIPs as weapons against cancer cells is either by designing molecules in which the toxic domains are linked to selective tumor targeting domains or directly delivered as suicide genes for cancer gene therapy. Here, we will provide a comprehensive picture of plant RIPs and discuss successful designs and features of chimeric molecules having therapeutic potential.

引用

页码：2699 / 2737

页数：39

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[32] SELF-POTENTIATION OF LIGAND-TOXIN CONJUGATES CONTAINING RICIN-A CHAIN FUSED WITH VIRAL STRUCTURES [J].