Development and validation of an LC-MS/MS method for the determination of six gestagens in kidney fats

被引：12

作者：

Rejtharova, Martina ^{[1
]}

Rejthar, Libor ^{[1
]}

机构：

[1] USKVBL Inst State Control Vet Biol & Med, Brno, Czech Republic

来源：

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT | 2013年 / 30卷 / 06期

关键词：

gestagens; fat tissue; LC-MS/MS; EXTRACTION; ACETYLGESTAGENS; STEROIDS;

D O I：

10.1080/19440049.2013.789555

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A method for the determination of residues of six steroids with gestagenic action (altrenogest, medroxyprogesterone acetate, megestrol acetate, melengestrol acetate, acetoxyprogesterone and chlormadinone acetate) in animal fat tissue was developed. The procedure consists of methanol extraction, clean-up on an alumina column and LC-MS/MS measurement. The method has been validated according to Decision 2002/657/EC. Decision limits for all analytes were observed within the range from 0.3 to 1.7ng g(-1), and recoveries were between 80% and 105%. The method is robust and can be applied for both screening and confirmatory analyses in routine residue monitoring.

引用

页码：995 / 999

页数：5

共 12 条

[1]

[Anonymous], 1996, Off. J. Eur. Commun, VL125, P3

[2]

[Anonymous], 2007, CRL GUID PAP CRLS VI

[3]

Commission Regulation (EU), 2010, Orkesterjournalen L, V15, P1

[4]

EC, 2003, Official Journal of the European Union, V333, P17

[5]

EC, 2002, OFFICIAL J EUROPEA L, P8

[6] Screening for gestagens in kidney fat using accelerated solvent extraction and liquid chromatography electrospray tandem mass spectrometry [J].

Hooijerink, H ;

van Bennekom, EO ;

Nielen, MWF .

ANALYTICA CHIMICA ACTA, 2003, 483 (1-2) :51-59

[7] Faster analysis of anabolic steroids in kidney fat by downscaling the sample size and using gas chromatography-tandem mass spectrometry [J].

Impens, S ;

Courtheyn, D ;

De Wasch, K ;

De Brabander, HF .

ANALYTICA CHIMICA ACTA, 2003, 483 (1-2) :269-280

[8] Determination of gestagens in kidney fat by liquid chromatography tandem mass spectrometry [J].

Lohmus, Madis ;

Kender, Tiia .

ANALYTICA CHIMICA ACTA, 2007, 586 (1-2) :233-238

[9] Confirmatory method for the determination of various acetylgestagens in animal kidney fat using liquid chromatography-tandem mass spectrometry [J].

Malone, Edward ;

Dowling, Geraldine ;

Elliott, Chris ;

Kennedy, Glenn ;

Regan, Liam .

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2009, 26 (05) :672-682

[10] Confirmatory analysis of steroids in muscle using liquid chromatography-tandem mass spectrometry [J].

Pedersen, Mikael ;

Andersen, Jens Hinge .

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2011, 28 (04) :428-437

← 1 2 →