Operation of pilot-scale nitrification-anammox reactors for the treatment of reject-water produced from the anaerobic digestion of thermal hydrolysis-treated sludge

Two-stage pilot-scale partial nitrification (PN)-anammox reactors were successfully operated for the treatment of reject-water (record as TRW) produced from the anaerobic digestion of thermal hydrolysis-treated sludge (THPAD). The PN reactor was operated stalely and Nitrosomonas was the major ammonia-oxidizing bacteria. In the anammox reactor, anammox activity doubled from day 3 to day 53 demonstrating that anammox adapted to the PN effluent. After acid shock at pH 4 for approximately 1 h, anammox was seriously inhibited and required approximately 60 days for recovery. This provided a reference for handling similar accidents. In the next 166 days, the load reached 0.40-0.51 kg N/(m(3).d) in the presence of high concentration COD (798-1313 mg/L), suggesting anammox can be used in high concentration organic wastewater. Under the combined action of anammox and denitrification, 94.7% nitrogen was removed. These results demonstrated TRW can be treated using PN-anammox technology which was conducive to the popularization of THPAD.