Testing Human Sperm Chemotaxis: How to Detect Biased Motion in Population Assays

被引：13

作者：

Armon, Leah ^{[1
]}

Caplan, S. Roy ^{[1
]}

Eisenbach, Michael ^{[1
]}

Friedrich, Benjamin M. ^{[2
,3
]}

机构：

[1] Weizmann Inst Sci, Dept Biol Chem, IL-76100 Rehovot, Israel

[2] Weizmann Inst Sci, Dept Mat & Interfaces, IL-76100 Rehovot, Israel

[3] Max Planck Inst Phys Komplexer Syst, Dresden, Germany

来源：

PLOS ONE | 2012年 / 7卷 / 03期

关键词：

HUMAN-SPERMATOZOA; FOLLICULAR FACTORS; CELLS; CHEMOATTRACTANT; PROGESTERONE; CAPACITATION; GUIDANCE; TRACKING; EGG;

D O I：

10.1371/journal.pone.0032909

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Biased motion of motile cells in a concentration gradient of a chemoattractant is frequently studied on the population level. This approach has been particularly employed in human sperm chemotactic assays, where the fraction of responsive cells is low and detection of biased motion depends on subtle differences. In these assays, statistical measures such as population odds ratios of swimming directions can be employed to infer chemotactic performance. Here, we report on an improved method to assess statistical significance of experimentally determined odds ratios and discuss the strong impact of data correlations that arise from the directional persistence of sperm swimming.

引用

页数：7

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