A label-free supersandwich electrogenerated chemiluminescence method for the detection of DNA methylation and assay of the methyltransferase activity

被引：53

作者：

Li, Yan ^{[1
]}

Luo, Xiaoe ^{[1
]}

Yan, Zhao ^{[1
]}

Zheng, Jianbin ^{[1
]}

Qi, Honglan ^{[2
]}

机构：

[1] NW Univ Xian, Inst Analyt Sci, Key Lab Electroanalyt Chem, Xian 710069, Shaanxi, Peoples R China

[2] Shaanxi Normal Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci Shaanxi Prov, Xian 710062, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 37期

关键词：

ELECTROCHEMILUMINESCENCE IMMUNOSENSOR; COLORIMETRIC ASSAY; COMPLEX MATRICES; NANOPARTICLES; HYPERMETHYLATION; UNIVERSAL; SYSTEM;

D O I：

10.1039/c3cc00203a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A method based on electrogenerated chemiluminescence (ECL) for detection of DNA methylation and assay of the methyltransferase activity is developed, and it is demonstrated that the label-free ECL method is capable of detecting methyltransferase with a detection limit of 3 x 10(-6) U mL(-1), using a supersandwich amplification technique.

引用

页码：3869 / 3871

页数：3

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