Lessons from human umbilical cord: gender differences in stem cells from Wharton's jelly

被引：21

作者：

Balzano, Francesca ^{[1
]}

Bellu, Emanuela ^{[1
]}

Basoli, Valentina ^{[1
,2
]}

Giudici, Silvia Dei ^{[3
]}

Santaniello, Sara ^{[1
,2
]}

Cruciani, Sara ^{[1
,2
]}

Facchin, Federica ^{[4
]}

Oggiano, Annalisa ^{[3
]}

Capobianco, Giampiero ^{[5
]}

Dessole, Francesco ^{[5
]}

Ventura, Carlo ^{[2
]}

Dessole, Salvatore ^{[5
]}

Maioli, Margherita ^{[1
,2
,6
,7
]}

机构：

[1] Univ Sassari, Dept Biomed Sci, Viale San Pietro 93-B, I-07100 Sassari, Italy

[2] CNR, Eldor Lab, Natl Inst Biostruct & Biosyst, Lab Mol Biol & Stem Cell Engn, Via Piero Gobetti 101, I-40129 Bologna, Italy

[3] Ist Zooprofilatt Sperimentale Sardegna, Via Vienna 2, I-07100 Sassari, Italy

[4] Univ Bologna, Dept Expt Diagnost & Special Med DIMES, Via Massarenti 9, I-40138 Bologna, Italy

[5] Univ Sassari, Gynecol & Obstet Clin, Dept Med Surg & Expt Sci, Sassari, Italy

[6] Univ Sassari, Dept Biomed Sci, Ctr Dev Biol & Reprogramming CEDEBIOR, Viale San Pietro 43-B, I-07100 Sassari, Italy

[7] CNR, Ist Ric Genet & Biomed, Cagliari, Italy

来源：

EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY | 2019年 / 234卷

关键词：

Stem cells; Wharton jelly; Gender; epigenetics; pluripotency; SELF-RENEWAL; EXPRESSION; DIFFERENTIATION; GENERATION; MOUSE; DNMT1; CYCLE; TOOL; SEX;

D O I：

10.1016/j.ejogrb.2018.12.028

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To study the molecular features of mesenchymal stem cells from Wharton Jelly (WJ-MSCs) of umbilical cord to predict their differentiation capacity. Design: Comparison of gene expression from mesenchymal stem cells of male and female umbilical cord Setting: University hospital Patient (s): umbilical cords (n = 12, 6 males and 6 females) retrieved from spontaneous full-term vaginal delivery of healthy women Intervention: we analyzed the expression of the sternness related genes C-MYC, OCT4, SOX2 and NANOG and of the epigenetic modulating gene DNA-methyltransferase 1 (DNMTI). Mean outcome measure: WJ-MSCs were isolated by standard procedures and immunophenotypically characterized. Gene expression analysis of sternness related genes and the epigenetic modulating gene DNMTI were performed by real-time PCR Results: expression of the OCT4 and DNMTI genes was significantly higher in WJ- MSCs isolated from male subjects, as compared to MSCs isolated from female-derived WJ. The resulting higher expression of OCT4 and DNMTI in WJ-MSCs from males as compared with female WJ-MSCs for the first time identifies a specific relationship between sternness genes, an epigenetic modulator, and gender differences. Conclusion: our findings disclose novel biomedical implications in WJ-MSCs related to the sex of the donor, thus providing additional cues to exploit their regenerative potential in allogenic transplantation. (C) 2019 Elsevier B.V. All rights reserved.

引用

页码：143 / 148

页数：6