Bone Marrow Endothelial Progenitors Augment Atherosclerotic Plaque Regression in a Mouse Model of Plasma Lipid Lowering

被引:48
作者
Yao, Longbiao [1 ]
Heuser-Baker, Janet [1 ]
Herlea-Pana, Oana [1 ]
Iida, Ryuji [2 ]
Wang, Qilong [3 ]
Zou, Ming-Hui [3 ]
Barlic-Dicen, Jana [1 ]
机构
[1] Oklahoma Med Res Fdn, Cardiovasc Biol Program, Oklahoma City, OK 73104 USA
[2] Oklahoma Med Res Fdn, Immunobiol & Canc Program, Oklahoma City, OK 73104 USA
[3] Univ Oklahoma, Hlth Sci Ctr, Dept Med, Div Mol Med, Oklahoma City, OK USA
关键词
Atherosclerosis regression; CXCR4 antagonist AMD3100; Bone marrow endothelial progenitors; COLONY-STIMULATING FACTOR; NITRIC-OXIDE; CXCR4; ANTAGONIST; HEMATOPOIETIC STEM; CELL RECRUITMENT; ARTERY-DISEASE; STATIN THERAPY; NILE RED; NEOVASCULARIZATION; AMD3100;
D O I
10.1002/stem.1256
中图分类号
Q813 [细胞工程];
学科分类号
摘要
The major event initiating atherosclerosis is hypercholesterolemia-induced disruption of vascular endothelium integrity. In settings of endothelial damage, endothelial progenitor cells (EPCs) are mobilized from bone marrow into circulation and home to sites of vascular injury where they aid endothelial regeneration. Given the beneficial effects of EPCs in vascular repair, we hypothesized that these cells play a pivotal role in atherosclerosis regression. We tested our hypothesis in the atherosclerosis-prone mouse model in which hypercholesterolemia, one of the main factors affecting EPC homeostasis, is reversible (Reversa mice). In these mice, normalization of plasma lipids decreased atherosclerotic burden; however, plaque regression was incomplete. To explore whether endothelial progenitors contribute to atherosclerosis regression, bone marrow EPCs from a transgenic strain expressing green fluorescent protein (GFP) under the control of endothelial cell-specific Tie2 promoter (Tie2-GFP(+)) were isolated. These cells were then adoptively transferred into atheroregressing Reversa recipients where they augmented plaque regression induced by reversal of hypercholesterolemia. Advanced plaque regression correlated with engraftment of Tie2-GFP(+) EPCs into endothelium and resulted in an increase in atheroprotective nitric oxide and improved vascular relaxation. Similarly augmented plaque regression was also detected in regressing Reversa mice treated with the stem cell mobilizer AMD3100 which also mobilizes EPCs to peripheral blood. We conclude that correction of hypercholesterolemia in Reversa mice leads to partial plaque regression that can be augmented by AMD3100 treatment or by adoptive transfer of EPCs. This suggests that direct cell therapy or indirect progenitor cell mobilization therapy may be used in combination with statins to treat atherosclerosis. STEM CELLS 2012; 30: 2720-2731
引用
收藏
页码:2720 / 2731
页数:12
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