A highly stable alkyl-amide silica-based column packing for reversed-phase HPLC of polar and ionizable compounds

Researchers have designed a new polar-linked, reversed-phase column for separating polar, ionizable, and especially highly basic compounds with excellent column efficiency and peak shapes. The column combines an ultrapure, low-acidity porous silica support with a unique stationary phase that has a polar, embedded amide group linking the sterically protecting diisopropyl groups with a C14 n-alkyl functionality, This new column demonstrates stability in low-pH mobile phases because of its resistance to hydrolysis and stationary-phase loss, Dense triple-endcapping minimizes unwanted silanol interactions and allows excellent peak shapes and stability at intermediate pH. The column can be used with totally aqueous mobile phases without the phase collapse typical of alkyl stationary phases.