Simultaneous determination of rutin and benzocaine in suppositories by reversed-phase high-performance liquid chromatography

被引：11
|
作者：
Joseph-Charles, J [1 ]
Montagut, M [1 ]
Langlois, MH [1 ]
Boyer, C [1 ]
Dubost, JP [1 ]
机构：
[1] Univ Bordeaux 2, UFR Sci Pharmaceut, Chim Analyt Lab, F-33076 Bordeaux, France
来源：
ANALYTICAL LETTERS | 2001年 / 34卷 / 15期
关键词：
rutin; benzocaine; high-performance liquid chromatography; simultaneous determination; suppositories;
D O I：
10.1081/AL-100108414
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A simple and precise reversed-phase HPLC procedure for the simultaneous determination of rutin and benzocaine in suppositories is proposed. The chromatographic conditions were: Apex ODS column; methanol-10mM triethylamine aqueous solution pH 3.0 (57:43, v/v) as the mobile phase; detection wavelength, 270 nm. Calibration curves were linear at concentration ranges 6.0 mug ml(-1)-14.0 mug ml(-1) and 1.5 mug ml(-1)-3.5 mug ml(-1) for rutin and benzocaine, respectively. The reproductibility was 1.05% and 0.95% for rutin and benzocaine, respectively.
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页码：2685 / 2692
页数：8
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