Simultaneous determination of rutin and benzocaine in suppositories by reversed-phase high-performance liquid chromatography

被引：11

作者：

Joseph-Charles, J ^{[1
]}

Montagut, M ^{[1
]}

Langlois, MH ^{[1
]}

Boyer, C ^{[1
]}

Dubost, JP ^{[1
]}

机构：

[1] Univ Bordeaux 2, UFR Sci Pharmaceut, Chim Analyt Lab, F-33076 Bordeaux, France

来源：

ANALYTICAL LETTERS | 2001年 / 34卷 / 15期

关键词：

rutin; benzocaine; high-performance liquid chromatography; simultaneous determination; suppositories;

D O I：

10.1081/AL-100108414

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and precise reversed-phase HPLC procedure for the simultaneous determination of rutin and benzocaine in suppositories is proposed. The chromatographic conditions were: Apex ODS column; methanol-10mM triethylamine aqueous solution pH 3.0 (57:43, v/v) as the mobile phase; detection wavelength, 270 nm. Calibration curves were linear at concentration ranges 6.0 mug ml(-1)-14.0 mug ml(-1) and 1.5 mug ml(-1)-3.5 mug ml(-1) for rutin and benzocaine, respectively. The reproductibility was 1.05% and 0.95% for rutin and benzocaine, respectively.

引用

页码：2685 / 2692

页数：8

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