Laser based enhancement of susceptibility of bacteria to antibiotic

被引：1

作者：

Reznick, Yana ^{[1
]}

Banin, Ehud ^{[2
]}

Lipovsky, Anat ^{[3
]}

Lubart, Rachel ^{[3
]}

Zalevsky, Zeev ^{[4
]}

机构：

[1] Bar Ilan Univ, Dept Phys, IL-52900 Ramat Gan, Israel

[2] Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel

[3] Bar Ilan Univ, Dept Chem, IL-52900 Ramat Gan, Israel

[4] Bar Ilan Univ, Fac Engn, IL-52900 Ramat Gan, Israel

来源：

BIOPHOTONICS AND IMMUNE RESPONSES VII | 2012年 / 8224卷

关键词：

Photodynamic therapy; Medical optics; Bacterial viability; Antimicrobial treatment; Laser; Gentamicin; IN-VITRO; VISIBLE-LIGHT; PORPHYROMONAS-GINGIVALIS; FUSOBACTERIUM-NUCLEATUM; STAPHYLOCOCCUS-AUREUS; MECHANISM;

D O I：

10.1117/12.901520

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Our objective is to test the effect of pulsed (Q-switched) and continuous wave (CW) laser light at wavelength of 532nm on the viability of free-living stationary phase bacteria with and without gentamicin (an antibiotic) treatment. Free living stationary phase gram negative bacteria (Pseudomonas aeruginosa strain PAO1) was immersed in Luria Broth (LB) solution and exposed to Q-switched and CW lasers with and without the addition of the antibiotic gentamicin. Cell viability was determined at different time points. Laser treatment alone did not reduce cell viability compared to untreated control and the gentamicin treatment alone only resulted in a 0.5 log reduction in the viable count for P. aeruginosa. The combined laser and gentamicin treatment, however, resulted in a synergistic effect and viability was reduced by 8 log's for P. aeruginosa PAO1.

引用

页数：10

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