Doping Polysulfone Membrane with Alpha-Tocopherol and Alpha-Lipoic Acid for Suppressing Oxidative Stress Induced by Hemodialysis Treatment

被引：14

作者：

Kohlova, Michaela ^{[1
,2
]}

Rocha, Susana ^{[1
]}

Gomes Amorim, Celia ^{[1
]}

de Nova Araujo, Alberto ^{[1
]}

Santos-Silva, Alice ^{[3
]}

Solich, Petr ^{[2
]}

Branco da Silva Montenegro, Maria Conceicao ^{[1
]}

机构：

[1] Univ Porto, Fac Pharm, Dept Chem Sci, LAQV REQUIMTE, Rua Jorge Viterbo Ferreira 228, P-4050313 Porto, Portugal

[2] Charles Univ Prague, Fac Pharm Hradec Kralove, Dept Analyt Chem, Akad Heyrovskeho 1203, Hradec Kralove 50005, Czech Republic

[3] Univ Porto, Fac Pharm, Dept Biol Sci, UCIBIO REQUIMTE, Rua Jorge Viterbo Ferreira 228, P-4050313 Porto, Portugal

来源：

MACROMOLECULAR BIOSCIENCE | 2020年 / 20卷 / 07期

关键词：

alpha-lipoic acid; alpha-tocopherol; hemodialysis; oxidative stress; polysulfone membranes; STAGE RENAL-DISEASE; E-COATED DIALYZER; VITAMIN-E; ASYMMETRIC MEMBRANE; PHASE-INVERSION; ASCORBIC-ACID; ANTIOXIDANT; INFLAMMATION; PERFORMANCE; BIOCOMPATIBILITY;

D O I：

10.1002/mabi.202000046

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The reduction of free radicals by bioactive membranes used for hemodialysis treatment is an important topic due to the constant rise of oxidative stress-associated cardiovascular mortality by hemodialysis patients. Therefore, it is urgent to find an effective solution that helps to solve this problem. Polysulfone membranes enriched with alpha-lipoic acid, alpha-tocopherol, and with both components are fabricated by spin coating. The antioxidant properties of these membranes are evaluated in vitro by determining the lipid-peroxidation level and the total antioxidant status of the blood plasma. The biocompatibility is assessed by quantifying the protein adsorption, platelet adhesion, complement activation, and hemolytic effect. All types of membranes show in vitro antioxidant activity and a trend to reduce oxidative stress in vivo; the best results show membranes prepared with a combination of both compounds and prove to be nonhemolytic and hemocompatible. Moreover, the membrane specific separation ability for the main waste products is not affected by antioxidants incorporation.

引用

页数：14

共 69 条

[31] Effects of phase inversion and rheological factors on formation of defect-free and ultrathin-skinned asymmetric polysulfone membranes for gas separation [J].