Silver nanoparticles protect human keratinocytes against UVB radiation-induced DNA damage and apoptosis: potential for prevention of skin carcinogenesis

被引:63
作者
Arora, Sumit [1 ]
Tyagi, Nikhil [1 ]
Bhardwaj, Arun [1 ]
Rusu, Lilia [1 ,2 ]
Palanki, Rohan [1 ]
Vig, Komal [3 ]
Singh, Shree R. [3 ]
Singh, Ajay P. [1 ,4 ]
Palanki, Srinivas [2 ]
Miller, Michael E. [5 ]
Carter, James E. [6 ]
Singh, Seema [1 ]
机构
[1] Univ S Alabama, Mitchell Canc Inst, Dept Oncol Sci, Mobile, AL 36688 USA
[2] Univ S Alabama, Dept Chem & Biomol Engn, Mobile, AL 36688 USA
[3] Alabama State Univ, Montgomery, AL 36101 USA
[4] Univ S Alabama, Coll Med, Dept Biochem & Mol Biol, Mobile, AL USA
[5] Auburn Univ, Res Instrumentat Facil, Adv Microscopy & Imaging Lab, Auburn, AL USA
[6] Univ S Alabama, Coll Med, Dept Pathol, Mobile, AL 36688 USA
基金
美国国家卫生研究院;
关键词
Silver nanoparticles; Ultraviolet radiations; DNA damage; Keratinocytes; Apoptosis; Nucleotide excision repair (NER); Cyclobutane pyrimidine dimers (CPDs); ULTRAVIOLET-RADIATION; TITANIUM-DIOXIDE; CELL-DEATH; CANCER; WAF1/CIP1; CYTOTOXICITY; ACTIVATION; SUNSCREENS; INDUCTION; TOXICITY;
D O I
10.1016/j.nano.2015.02.024
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Ultraviolet (UV)-B radiation from the sun is an established etiological cause of skin cancer, which afflicts more than a million lives each year in the United States alone. Here, we tested the chemopreventive efficacy of silver-nanoparticles (AgNPs) against UVB-irradiation-induced DNA damage and apoptosis in human immortalized keratinocytes (HaCaT). AgNPs were synthesized by reduction-chemistry and characterized for their physicochemical properties. AgNPs were well tolerated by HaCaT cells and their pretreatment protected them from UVB-irradiation-induced apoptosis along with significant reduction in cyclobutane-pyrimidine-dimer formation. Moreover, AgNPs pretreatment led to G1-phase cell-cycle arrest in UVB-irradiated HaCaT cells. AgNPs were efficiently internalized in UVB-irradiated cells and localized into cytoplasmic and nuclear compartments. Furthermore, we observed an altered expression of various genes involved in cell-cycle, apoptosis and nucleotide-excision repair in HaCaT cells treated with AgNPs prior to UVB-irradiation. Together, these findings provide support for potential utility of AgNPs as novel chemopreventive agents against UVB-irradiation-induced skin carcinogenesis. From the Clinical Editor: Excessive exposure to the sun is known to increase the risk of skin cancer due to DNA damage. In this work, the authors tested the use of silver nanoparticles as protective agents against ultraviolet radiation. The positive results may open a door for the use of silver nanoparticle as novel agents in the future. Published by Elsevier Inc.
引用
收藏
页码:1265 / 1275
页数:11
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