A polymerase chain reaction-based test for the identification of Trichoderma harzianum biotypes 2 and 4, responsible for the worldwide green mold epidemic in cultivated Agaricus bisporus

被引：21

作者：

Chen, X ^{[1
]}

Romaine, CP ^{[1
]}

Ospina-Giraldo, MD ^{[1
]}

Royse, DJ ^{[1
]}

机构：

[1] Penn State Univ, Dept Plant Pathol, University Pk, PA 16802 USA

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1999年 / 52卷 / 02期

关键词：

D O I：

10.1007/s002530051516

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe a polymerase chain reaction (PCR)-based test that is specific for the pathogenic European biotype 2 (Th2) and North American biotype 4 (Th4) of Trichoderma harzianum, responsible for the green mold epidemic in the cultivated mushroom, Agaricus bisporus. A PCR primer pair was designed that targets a 444-bp arbitrary sequence in the genome of Th4. The primers also amplified the same product with Th2, but showed no reactivity with other biotypes of T. harzianum, several biocontrol Trichoderma, or with 31 other genera and species of fungi. The PCR-based test should have application in disease management programs, and in the evaluation of biocontrol Trichoderma for potential pathogenicity on mushrooms.

引用

页码：246 / 250

页数：5

共 18 条

[1]

Ausubel FM, 1995, SHORT PROTOCOLS MOL

[2]

Castle A, 1998, APPL ENVIRON MICROB, V64, P133

[3]

CHEN X, 1999, IN PRESS APPL ENV MI

[4]

Doyle J. J., 1987, FOCUS, V19, P11, DOI DOI 10.2307/2419362

[5] A NUCLEOTIDE SUBSTITUTION IN ONE OF THE BETA-TUBULIN GENES OF TRICHODERMA-VIRIDE CONFERS RESISTANCE TO THE ANTIMITOTIC DRUG METHYL BENZIMIDAZOLE-2-YL-CARBAMATE [J].

GOLDMAN, GH ;

TEMMERMAN, W ;

JACOBS, D ;

CONTRERAS, R ;

VANMONTAGU, M ;

HERRERAESTRELLA, A .

MOLECULAR & GENERAL GENETICS, 1993, 240 (01) :73-80

[6] Physiological and biochemical characterization of Trichoderma harzianum, a biological control agent against soilborn fungal plant pathogens [J].

Grondona, I ;

Hermosa, R ;

Tejada, M ;

Gomis, MD ;

Mateos, PF ;

Bridge, PD ;

Monte, E ;

GarciaAcha, I .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (08) :3189-3198

[7]

MORRIS E, 1995, SCIENCE AND CULTIVATION OF EDIBLE FUNGI, VOLS 1 AND 2, P611

[8] INTRASPECIFIC MOLECULAR VARIATION AMONG TRICHODERMA-HARZIANUM ISOLATES COLONIZING MUSHROOM COMPOST IN THE BRITISH-ISLES [J].

MUTHUMEENAKSHI, S ;

MILLS, PR ;

BROWN, AE ;

SEABY, DA .

MICROBIOLOGY-SGM, 1994, 140 :769-777

[9] Phylogenetic relationships of Trichoderma harzianum causing mushroom green mold in Europe and North America to other species of Trichoderma from world-wide sources [J].

Ospina-Giraldo, MD ;

Royse, DJ ;

Thon, MR ;

Chen, X ;

Romaine, CP .

MYCOLOGIA, 1998, 90 (01) :76-81

[10]

OSPINAGIRALDO MD, 1999, IN PRESS PHYTOPATHOL

← 1 2 →