Fluoroimmunoassays for the detection of zearalenone in maize using CdTe/CdS/ZnS quantum dots

被引：66
作者：
Zhang, Fuyuan [1 ,3 ]
Liu, Bing [1 ]
Sheng, Wei [1 ]
Zhang, Yan [1 ]
Liu, Qi [1 ]
Li, Shijie [1 ]
Wang, Shuo [1 ,2 ]
机构：
[1] Tianjin Univ Sci & Technol, Key Lab Food Nutr & Safety, Minist Educ China, Tianjin 300457, Peoples R China
[2] Beijing Technol & Business Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100048, Peoples R China
[3] Macquarie Univ, ARC Ctr Excellence Nanoscale Biophoton CNBP, N Ryde, NSW 2109, Australia
来源：
FOOD CHEMISTRY | 2018年 / 255卷
基金：
中国国家自然科学基金;
关键词：
Zearalenone; Quantum dots; CdTe/CdS/ZnS; Fluoroimmunoassay; Immunochromatographic strip; LINKED-IMMUNOSORBENT-ASSAY; IMMUNOCHROMATOGRAPHIC ASSAY; MONOCLONAL-ANTIBODY; SENSITIVE DETECTION; MYCOTOXIN DETECTION; BROAD-SPECIFICITY; MASS-SPECTROMETRY; IMMUNOASSAY; CEREALS; SAMPLES;
D O I：
10.1016/j.foodchem.2018.02.060
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
CdTe/CdS/ZnS quantum dots (QDs) were synthesized in aqueous phase and conjugated with a new anti-zearalenone (ZEN) monoclonal antibody. Using this novel fluorescent probe, a fluoroimmunoassay (FLISA) and a rapid immunochromatographic strip (ICTS) were developed for the detection of ZEN in maize. Our proposed FLISA allowed for ZEN determination in the range of 0.038-0.977 ng/mL with an IC50 of 0.162 ng/mL and a limit of detection (LOD) of 0.012 ng/mL occurring in maize. The rapid ICTS had a visual LOD of 1.0 ng/mL in buffer solution and 1.5 ng/mL in maize extract. These two QDs-based immunoassays were all successfully verified by commercial ELISA kits. The results confirmed that: firstly, the FLISA can be used as a robust method for the sensitive detection of ZEN; and secondly, the ICTS is ideally suited for rapidly screening large numbers of samples.
引用
收藏
页码：421 / 428
页数：8
相关论文
共 35 条
  • [1] Investigation of an Immunoassay with Broad Specificity to Quinolone Drugs by Genetic Algorithm with Linear Assignment of Hypermolecular Alignment of Data Sets and Advanced Quantitative Structure-Activity Relationship Analysis
    Chen, Jiahong
    Lu, Ning
    Shen, Xing
    Tang, Qiushi
    Zhang, Chijian
    Xu, Jun
    Sun, Yuanming
    Huang, Xin-an
    Xu, Zhenlin
    Leit, Hongtao
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2016, 64 (13) : 2772 - 2779
  • [2] A fluorescent immunochromatographic strip test using Quantum Dots for fumonisins detection
    Di Nardo, F.
    Anfossi, L.
    Giovannoli, C.
    Passini, C.
    Goftman, V. V.
    Goryacheva, I. Y.
    Baggiani, C.
    [J]. TALANTA, 2016, 150 : 463 - 468
  • [3] Quantum-DoT submicrobead-based immunochromatographic assay for quantitative and sensitive detection of zearalenone
    Duan, Hong
    Chen, Xuelan
    Xu, Wei
    Fu, Jinhua
    Xiong, Yonghua
    Wang, Andrew
    [J]. TALANTA, 2015, 132 : 126 - 131
  • [4] Development of enzyme-linked immunosorbent assay for determination of polybrominated diphenyl ether BDE-121
    Feng, Hongyan
    Zhou, Liping
    Shi, Lei
    Li, Weili
    Yuan, Lijuan
    Li, Daqing
    Cai, Qingyun
    [J]. ANALYTICAL BIOCHEMISTRY, 2014, 447 : 49 - 54
  • [5] Comparative study of colloidal gold and quantum dots as labels for multiplex screening tests for multi-mycotoxin detection
    Foubert, Astrid
    Beloglazova, Natalia V.
    De Saeger, Sarah
    [J]. ANALYTICA CHIMICA ACTA, 2017, 955 : 48 - 57
  • [6] A Quantum Dot-Based Immunoassay for Screening of Tetracyclines in Bovine Muscle
    Garcia-Fernandez, Jenifer
    Trapiella-Alfonso, Laura
    Costa-Fernandez, Jose M.
    Pereiro, Rosario
    Sanz-Medel, Alfredo
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2014, 62 (07) : 1733 - 1740
  • [7] Synthesis, modification, bioconjugation of silica coated fluorescent quantum dots and their application for mycotoxin detection
    Goftman, Valentina V.
    Aubert, Tangi
    Vande Ginste, Dries
    Van Deun, Rik
    Beloglazova, Natalia V.
    Hens, Zeger
    De Saeger, Sarah
    Goryacheva, Irina Yu
    [J]. BIOSENSORS & BIOELECTRONICS, 2016, 79 : 476 - 481
  • [8] A rapid method for simultaneous determination of zearalenone, α-zearalenol, β-zearalenol, zearalanone, α-zearalanol and β-zearalanol in traditional Chinese medicines by ultra-high-performance liquid chromatography-tandem mass spectrometry
    Han, Zheng
    Ren, Yiping
    Zhou, Hailong
    Luan, Lianjun
    Cai, Zengxuan
    Wu, Yongjiang
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2011, 879 (5-6): : 411 - 420
  • [9] Simplified calibration and analysis on screen-printed disposable platforms for electrochemical magnetic bead-based inmunosensing of zearalenone in baby food samples
    Hervas, Mirian
    Angel Lopez, Miguel
    Escarpa, Alberto
    [J]. BIOSENSORS & BIOELECTRONICS, 2010, 25 (07) : 1755 - 1760
  • [10] Cell labeling and cytotoxicity of aqueously synthesized CdTe/CdS/ZnS core-shell-shell quantum dots by a water bath-hydrothermal method
    Huang, Huaiqing
    Liu, Jinling
    Han, Baofu
    Mi, Congcong
    Xu, Shukun
    [J]. JOURNAL OF LUMINESCENCE, 2012, 132 (04) : 1003 - 1009
1234