Detection of suppressed maturation of the human COQ5 protein in the mitochondria following mitochondrial uncoupling by an antibody recognizing both precursor and mature forms of COQ5

被引：12
作者：
Chen, Shih-Wei [1 ,2 ]
Liu, Chia-Chi [1 ]
Yen, Hsiu-Chuan [1 ,2 ]
机构：
[1] Chang Gung Univ, Coll Med, Dept Med Biotechnol & Lab Sci, Tao Yuan 333, Taiwan
[2] Chang Gung Univ, Coll Med, Grad Inst Biomed Sci, Div Biotechnol, Tao Yuan 333, Taiwan
来源：
MITOCHONDRION | 2013年 / 13卷 / 02期
关键词：
Coenzyme Q(10); Mitochondrial targeting signal; Protein purification; Polyclonal antibody; Chemical uncoupler; Knockdown; COENZYME-Q; SACCHAROMYCES-CEREVISIAE; C-METHYLTRANSFERASE; OXIDATIVE STRESS; PURIFICATION; IMPORT; CARDIOMYOPATHY; BIOSYNTHESIS; Q(10); GENE;
D O I：
10.1016/j.mito.2013.01.007
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Yeast Coq5p is required for the biosynthesis of coenzyme Q(6) (CoQ(6)), but its human homolog has not been studied. We purified soluble recombinant human COQ5 protein under native conditions and generated an antibody recognizing both precursor and mature forms of COQ5. Mitochondrial localization of the mature form in 143B cells was demonstrated with this antibody. Moreover, a chemical uncoupler in a dose that suppressed CoQ(10) levels downregulated the mature form but augmented the precursor form of COQ5. The results that knockdown of the COQ5 gene reduced CoQ(10) levels further indicated the critical role of COQ5 in the biosynthesis of CoQ(10). (C) 2013 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
引用
收藏
页码：143 / 152
页数：10
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