Measuring lateral efficiency of optical traps: The effect of tube length

被引：22

作者：

Reihani, SNS ^{[1
]}

Khalesifard, HR ^{[1
]}

Golestanian, R ^{[1
]}

机构：

[1] Inst Adv Studies Basic Studies, Dept Phys, Zanjan 451951159, Iran

来源：

OPTICS COMMUNICATIONS | 2006年 / 259卷 / 01期

关键词：

optical trap; trap efficiency; spherical aberration; tube length;

D O I：

10.1016/j.optcom.2005.08.018

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

An optical tweezer setup is made based on a custom-designed inverted microscope, which can work both in finite and infinite tube length microscopy modes. It is shown that the spherical aberration due to the mismatch in the refractive indices of the specimen (water) and the immersion oil as well as the wavelength can be partially compensated by introducing another source for the spherical aberration provided it has the opposite sign. Changing the tube length is shown to be a good candidate for this effect: an improvement of up to a factor of four has been observed in the lateral efficiency of the trap. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：204 / 211

页数：8

共 13 条

[1] Two-dimensional tracking of ncd motility by back focal plane interferometry [J].

Allersma, MW ;

Gittes, F ;

deCastro, MJ ;

Stewart, RJ ;

Schmidt, CF .

BIOPHYSICAL JOURNAL, 1998, 74 (02) :1074-1085

[2] FORCES OF A SINGLE-BEAM GRADIENT LASER TRAP ON A DIELECTRIC SPHERE IN THE RAY OPTICS REGIME [J].

ASHKIN, A .

BIOPHYSICAL JOURNAL, 1992, 61 (02) :569-582

[3] Single-molecule studies of DNA mechanics [J].

Bustamante, C ;

Smith, SB ;

Liphardt, J ;

Smith, D .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2000, 10 (03) :279-285

[4] Interference model for back-focal-plane displacement detection in optical tweezers [J].

Gittes, F ;

Schmidt, CF .

OPTICS LETTERS, 1998, 23 (01) :7-9

[5] Stretching biological cells with light [J].

Guck, J ;

Ananthakrishnan, R ;

Cunningham, CC ;

Käs, J .

JOURNAL OF PHYSICS-CONDENSED MATTER, 2002, 14 (19) :4843-4856

[6] Characterization of trapping force in the presence of spherical aberration [J].

Ke, PC ;

Gu, M .

JOURNAL OF MODERN OPTICS, 1998, 45 (10) :2159-2168

[7] Optical trapping [J].

Neuman, KC ;

Block, SM .

REVIEW OF SCIENTIFIC INSTRUMENTS, 2004, 75 (09) :2787-2809

[8] The motion of a single molecule, the λ-receptor, in the bacterial outer membrane [J].

Oddershede, L ;

Dreyer, JK ;

Grego, S ;

Brown, S ;

Berg-Sorenson, K .

BIOPHYSICAL JOURNAL, 2002, 83 (06) :3152-3161

[9] Trapping forces, force constants, and potential depths for dielectric spheres in the presence of spherical aberrations [J].

Rohrbach, A ;

Stelzer, EHK .

APPLIED OPTICS, 2002, 41 (13) :2494-2507

[10] INFLUENCE OF SPHERICAL-ABERRATION ON AXIAL IMAGING OF CONFOCAL REFLECTION MICROSCOPY [J].

SHEPPARD, CJR ;

GU, M ;

BRAIN, K ;

ZHOU, H .

APPLIED OPTICS, 1994, 33 (04) :616-624

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