Osteoblast differentiation of umbilical cord blood-derived mesenchymal stem cells and enhanced cell adhesion by fibronectin

被引：13

作者：

Mekala, Naveen Kumar ^{[1
]}

Baadhe, Rama Raju ^{[1
]}

Parcha, Sreenivasa Rao ^{[1
]}

Devi, Prameela Y. ^{[2
]}

机构：

[1] Natl Inst Technol, Dept Biotechnol, Warangal 506004, Andhra Pradesh, India

[2] Kakatiya Univ, Dept Zool, Warangal 506009, Andhra Pradesh, India

来源：

TISSUE ENGINEERING AND REGENERATIVE MEDICINE | 2012年 / 9卷 / 05期

关键词：

cord blood cells; fibronectin; mesenchymal stem cells; osteogenic differentiation; tissue engineering; BONE; GROWTH;

D O I：

10.1007/s13770-012-0359-z

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Mononuclear cells isolated from human umbilical cord blood were differentiated in to spindle shaped fibrous mesenchymal stem cells (MSCs) in DMEM with 10% fetal bovine serum. These progenitor cells were further examined for their ability to differentiate into osteoblasts by culturing them in osteogenic differentiation media (Stem ProA (R)). Calcium mineralization assay, alizarin red S, von kossa staining confirmed that MSCs from cord blood were capable of mineralization when they were cultured in osteogenic medium. The adhesion studies shown that MSCs have greater affinity towards fibronectin (FN) coated dishes, compared to albumin (Alb) coated dishes due to CD29and CD44 surface receptors.

引用

页码：259 / 264

页数：6

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