Real-time PCR assay for the discrimination and quantification of wheat and barley strains of Wheat dwarf virus

被引：27

作者：

Gadiou, S. ^{[1
]}

Ripl, J. ^{[1
]}

Janourova, B. ^{[1
]}

Jarosova, J. ^{[1
]}

Kundu, J. K. ^{[1
]}

机构：

[1] Res Inst Crop Prod, Div Plant Hlth, Dept Virol, CR-16106 Prague, Czech Republic

来源：

VIRUS GENES | 2012年 / 44卷 / 02期

关键词：

qPCR; WDV; Quantification; RT-PCR; AMPLIFICATION; GEMINIVIRUSES; SEQUENCE; TAQMAN;

D O I：

10.1007/s11262-011-0699-0

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A rapid method for detection, discrimination and quantification of wheat and barley strains of wheat dwarf virus (WDV) was successfully developed. The sensitivity of quantification of the wheat and barley strains of WDV ranged from an average of 1.2 x 10(7)-1.2 x 10(2) and from an average of 1.4 x 10(7)-1.4 x 10(4) copies of viral genome, respectively. These standard serial dilutions were applied to plant and vector tissues for virus titer calculations. Both strains of WDV were clearly discriminated by specific probes and melting curve analysis. Both TaqMan(A (R)) and SYBRA (R) Green technologies provided accurate and reliable methods for monitoring, detection, discrimination, and quantification of WDV.

引用

页码：349 / 355

页数：7

共 29 条

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