Ultrasound localization microscopy of renal tumor xenografts in chicken embryo is correlated to hypoxia

被引:53
作者
Lowerison, Matthew R. [1 ,2 ,3 ]
Huang, Chengwu [3 ]
Lucien, Fabrice [4 ]
Chen, Shigao [3 ]
Song, Pengfei [1 ,2 ,3 ]
机构
[1] Univ Illinois, Beckman Inst Adv Sci & Technol, Urbana, IL 61801 USA
[2] Univ Illinois, Dept Elect & Comp Engn, Urbana, IL 61801 USA
[3] Mayo Clin, Dept Radiol, Coll Med & Sci, Rochester, MN 55905 USA
[4] Mayo Clin, Dept Urol, Coll Med & Sci, Rochester, MN USA
基金
美国国家卫生研究院;
关键词
CONTRAST-ENHANCED ULTRASOUND; CANCER; VASCULATURE; TOMOGRAPHY; SUPERRESOLUTION; NORMALIZATION; ANGIOGENESIS; MECHANISMS; TORTUOSITY; THERAPY;
D O I
10.1038/s41598-020-59338-z
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Ultrasound localization microscopy (ULM) permits the reconstruction of super-resolved microvascular images at clinically relevant penetration depths, which can be potentially leveraged to provide noninvasive quantitative measures of tissue hemodynamics and hypoxic status. We demonstrate that ULM microbubble data processing methods, applied to images acquired with a Verasonics Vantage 256 system, can provide a non-invasive imaging surrogate biomarker of tissue oxygenation status. This technique was applied to evaluate the microvascular structure, vascular perfusion, and hypoxia of a renal cell carcinoma xenograft model grown in the chorioallantoic membrane of chicken embryos. Histological microvascular density was significantly correlated to ULM measures of intervessel distance (R = -0.92, CI95 = [-0.99,-0.42], p = 0.01). The Distance Metric, a measure of vascular tortuosity, was found to be significantly correlated to hypoxyprobe quantifications (R = 0.86, CI95 = [0.17, 0.99], p = 0.03). ULM, by providing non-invasive in vivo microvascular structural information, has the potential to be a crucial clinical imaging modality for the diagnosis and therapy monitoring of solid tumors.
引用
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页数:13
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